慢性乙型病毒性肝炎脾虚湿热证潜在microRNA表达谱研究

发布时间：2019-02-21 16:32

【摘要】：目的:筛选慢性乙型病毒性肝炎(CHB)脾虚湿热证的潜在microRNA表达谱,探讨CHB脾虚湿热证的生物学标志物。方法:运用基因芯片技术检测CHB脾虚湿热证组、隐证组及健康对照组的microRNA,通过数据分析,选择脾虚湿热证组中与隐证组和健康对照组比较具有显著差异的microRNA作为其潜在的microRNA表达谱。并通过靶基因预测及富集分析等进行功能分析,探讨差异microRNA表达谱影响证候形成的可能路径。结果:研究获得miR-378a-3p等17条microRNA在脾虚湿热证组与隐证组、健康对照组间存在显著差异,可作为CHB脾虚湿热证的潜在microRNA表达谱。结论:CHB脾虚湿热证的潜在microRNA表达谱为:miR-378a-3p、miR-4745-5p、miR-1275、miR-423-5p、miR-3188、miR-4800-5p、miR-4455、miR-939、miR-3676-5p、miR-3135b、miR-3141、miR-1587、miR-494、miR-4707-5p、miR-4739、miR-197-3p、miR-320a,其调控机制可能与核心启动子、MAPK、PI3K/Akt信号通路等有关。
[Abstract]:Objective: to screen the potential microRNA expression profile of (CHB) spleen deficiency dampness heat syndrome in chronic hepatitis B and to explore the biomarkers of CHB spleen deficiency dampness heat syndrome. Methods: the microRNA, of CHB spleen deficiency dampness heat syndrome group, hidden syndrome group and healthy control group were detected by using gene chip technology through data analysis. The potential microRNA expression profile of spleen deficiency dampness heat syndrome group was selected as microRNA which had significant difference compared with hidden syndrome group and healthy control group. The functional analysis of target gene prediction and enrichment analysis was carried out to explore the possible pathway of differential microRNA expression profile affecting the formation of syndromes. Results: 17 microRNA such as miR-378a-3p were obtained in the spleen deficiency dampness heat syndrome group, the hidden syndrome group and the healthy control group, and there was significant difference between the two groups, which could be used as the potential microRNA expression profile of the spleen deficiency dampness heat syndrome of CHB. Conclusion: the potential microRNA expression profile of spleen deficiency and dampness heat syndrome in CHB is as follows: miR-378a-3p,miR-4745-5p,miR-1275,miR-423-5p,miR-3188,miR-4800-5p,miR-4455,miR-939,miR-3676-5p,miR-3135b,miR-3141,miR-1587, The regulatory mechanism of miR-494,miR-4707-5p,miR-4739,miR-197-3p,miR-320a, may be related to core promoter, MAPK,PI3K/Akt signaling pathway and so on.
【作者单位】：成都中医药大学;雅安市中医医院;
【基金】：“十二五”传染病国家科技重大专项(No.2012ZX10005001)~~
【分类号】：R259

【相似文献】