从RhoA通路探讨电针改善脑缺血再灌注大鼠神经功能的机制

发布时间：2019-02-28 16:45

【摘要】：【目的】探讨电针对大脑中动脉闭塞(MCAO)缺血再灌注(I/R)大鼠神经功能的影响及潜在机制,为应用电针治疗急性脑梗死提供实验依据。【方法】将54只雄性SPF级SD大鼠采用线栓法复制MCAO模型,按照随机数字表法分为假手术组、脑梗死组、电针组,每组18只大鼠。假手术组仅做手术创伤,脑梗死组不予任何干预,电针组予电针针刺百会穴、大椎穴,每天1次,每次30 min。各时间点(第1、7、14天)采用神经功能缺损评分量表评定神经功能,蛋白免疫印迹法(Western blot)检测病灶侧海马区勿动蛋白-A(Nogo-A)、Ras同源基因家族成员A(RhoA)、溶血磷脂酸(LPA)蛋白的表达。【结果】第1、7、14天脑梗死组神经功能缺损积分、海马区Nogo-A及LPA蛋白的表达均显著高于假手术组(P0.05),第7、14天脑梗死组病灶侧海马区RhoA蛋白的表达显著高于假手术组(P0.05);电针组第1、7、14天神经功能缺损积分、病灶侧海马区RhoA、Nogo-A及LPA蛋白的表达均显著低于同时间点脑梗死组(P0.05)。【结论】电针可改善大鼠脑梗死后神经功能,其机制可能与电针抑制大鼠海马区RhoA通路相关蛋白的表达密切相关。
[Abstract]:[objective] to investigate the effects of electroacupuncture on the neural function of middle cerebral artery occlusion (MCAO) rats with (MCAO) ischemia-reperfusion (I / R) and its potential mechanism. [methods] 54 male SPF grade SD rats were randomly divided into sham operation group, cerebral infarction group and electroacupuncture group with 18 rats in each group according to the method of random numerical table, and 54 male SD rats with acute cerebral infarction were divided into sham operation group, cerebral infarction group and electro acupuncture group with 18 rats in each group. Sham operation group only do operation trauma, cerebral infarction group without any intervention, electroacupuncture group to acupuncture Baihui point, Dazhui point, once a day, 30 min. each time. At each time point (1,7,14 days), the neurologic function was assessed by the nerve function defect scale, and the A (RhoA), (A (RhoA), a member of the Nogo-A), Ras homologue gene family) was detected by protein immunoblotting (Western blot) in the focal side of the hippocampus. [results] the expression of lysophosphatidic acid (LPA) protein in cerebral infarction group was significantly higher than that in sham operation group on day 1,7,14 (P0.05), and the expression of Nogo-A and LPA protein in hippocampus of cerebral infarction group was significantly higher than that in sham operation group (P0.05). On the 7th and 14th day, the expression of RhoA protein in cerebral infarction group was significantly higher than that in sham operation group (P0.05). On the 1st, 7th and 14th day, the nerve function defect score, the expression of RhoA,Nogo-A and LPA protein in the hippocampus of the acupuncture group were significantly lower than those in the cerebral infarction group at the same time point (P0.05). [conclusion] electroacupuncture can improve the nerve function after cerebral infarction in rats. The mechanism may be related to the inhibitory effect of electroacupuncture on the expression of RhoA pathway-related proteins in hippocampus of rats.
【作者单位】：广东省佛山市中医院;南方医科大学南方医院;
【基金】：国家自然科学基金课题资助项目(编号:81473470,81072947) 广东省自然科学基金重点项目(编号:8152800007000001,2014A030311033)
【分类号】：R245.97

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