神阙灸法治疗EAT模型小鼠的疗效及机制初探

发布时间：2019-03-26 13:32

【摘要】：目的:观察神阙灸法对实验性自身免疫性甲状腺炎(EAT)模型小鼠的疗效并探讨可能机制。方法:运用猪甲状腺球蛋白(PTg)皮下多点免疫联合饮用高碘水的方法制作小鼠EAT模型,并随机分为空白组、模型组、治疗组。采用酶联免疫吸附法(ELISA)检测小鼠促甲状腺素(TSH)、甲状腺球蛋白抗体(TGAb)水平;苏木素-伊红染色(HE)观察甲状腺及神阙穴局部皮肤组织形态;免疫组织化学法检测甲状腺、神阙穴局部皮肤组织中的转化生长因子-β1(TGF-β1)、瞬时受体电位香草酸亚家族-1(TRPV1)水平。结果:与模型组相比,治疗组小鼠甲状腺组织充血、水肿、滤泡上皮变性坏死和炎症细胞浸润均较轻,甲状腺球蛋白合成正常,且小鼠血清TGAb水平显著下降(P0.05),神阙穴局部皮肤组织TGF-β1、TRPV1表达增强(P0.05),而血清TSH、甲状腺组织TGF-β1、TRPV1水平未见显著差异(P0.05)。结论:神阙灸法能够显著减轻EAT小鼠甲状腺组织炎症反应,其疗效可能与神阙灸法激活局部组织TGF-β1、TRPV1表达,继而抑制免疫反应,使EAT小鼠甲状腺自身抗体水平下降有关。
[Abstract]:Objective: to observe the therapeutic effect of Shenque moxibustion on experimental autoimmune thyroiditis (EAT) in mice and to explore the possible mechanism. Methods: the EAT model of mice was made by subcutaneous multipoint immunization with pig thyroglobulin (PTg) combined with drinking high iodine water. The mice were randomly divided into three groups: blank group, model group and treatment group. The (TGAb) level of thyrotropin (TSH), thyroglobulin antibody in mice was detected by enzyme-linked immunosorbent assay (ELISA), and the local skin tissue morphology of thyroid and Shenque point was observed by hematoxylin-eosin staining (HE). The levels of transforming growth factor-尾 1 (TGF- 尾 1) and transient receptor potential vanillic acid subfamily-1 (TRPV1) in the skin of thyroid and Shenque acupoints were detected by immunohistochemistry. Results: compared with the model group, the hyperemia, edema, degeneration and necrosis of follicular epithelium and infiltration of inflammatory cells in the treatment group were mild, thyroglobulin synthesis was normal, and serum TGAb level was significantly decreased (P0.05). The expression of TGF- 尾 1 and TRPV1 in local skin tissue of Shenque point was increased (P0.05), but the levels of TGF- 尾 1 and TRPV1 in serum TSH, thyroid tissue were not significantly different (P0.05). Conclusion: Shenque moxibustion can significantly reduce the inflammation of thyroid tissue in EAT mice, and its therapeutic effect may be related to the activation of TGF- 尾 1 and TRPV1 expression in local tissues by Shenque moxibustion method, and then the inhibition of immune response, resulting in the decrease of thyroid autoantibody level in EAT mice.
【作者单位】：东南大学医学院;东南大学附属中大医院针灸科;
【基金】：国家自然科学基金资助项目(81603695)
【分类号】：R245;R-332

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