海洛因或冰毒成瘾者外周血白细胞端粒长度与吸毒年限、中医证素关系的研究

发布时间：2019-04-08 14:29

【摘要】：目的：探讨海洛因成瘾者和冰毒成瘾者的外周血白细胞端粒长度与中医证素的关系,从而在基因水平上探讨海洛因和冰毒对人体健康影响的可能中医内在机制。方法：研究对象均为年龄在20-49岁的汉族人。实验组为342例毒品吸食者,其中海洛因吸食者206例(男性129例和女性77例),冰毒吸食者136例(男性80例和女性56例),均为来源于2012年-2014年在福州市公安强制戒毒所和司法厅戒毒管理局所辖的强制戒毒所自愿参加调查的戒毒人员。对照组为招募自福建省第二人民医院体检中心的200例健康受试者(男性115例和女性85例)。规范采集四诊信息,应用证素辨证方法提取中医证素特征,运用QPCR基础技术测量外周血白细胞的端粒长度。结果：1.健康组、海洛因组及冰毒组的年龄均为20-49岁,且三组年龄、性别分布无差异(P0.05)。所有参与者的白细胞端粒长度与年龄、性别呈显著负相关(P0.05)；校正年龄和性别后,海洛因组和冰毒组的端粒长度均比健康组的端粒长度短(P0.05)。2.校正年龄和性别后,海洛因组和冰毒组间端粒长度无差异(P0.05)；两组端粒长度与吸毒年限均无明显相关性(P0.05)；两组内各吸毒年限的端粒长度无明显差异(P0.05)。3.海洛因和冰毒成瘾者的中医证素分布大致相同,病位均以肝、脾、肾为主,尤以肝最为多见；病性上,实证以气滞最为多见；虚证以气虚最为多见。4.校正年龄和性别后,观察端粒长度变化与中医证素间的关系,发现形成各证素的海洛因组中的端粒长度与健康组的端粒长度均没有明显差异(P0.05)；形成肝、湿、热等证素的冰毒组端粒长度与健康组的端粒长度之间均具有明显差异(P0.05),且端粒长度比健康组的端粒长度短。5.海洛因组在吸毒年限为4-12年时,20-29岁与30-39岁两个年龄段的端粒长度有显著差异(P0.01),且30-39岁年龄段的端粒长度较20-29岁年龄段的端粒长度缩短；两年龄段间,肝、脾、肾、气滞、湿、痰、气虚、阳虚及血虚等证素的端粒长度有明显差异(P0.05)。结论：1.吸食海洛因或吸食冰毒均会造成吸毒人员外周血白细胞端粒的损伤,使端粒长度缩短。2.吸食海洛因或吸食冰毒均会引起脏腑功能失调及气血津液损伤,病位主要在肝、肾、脾,尤其以肝的功能失调最为明显；气滞、气虚是海洛因和冰毒成瘾者最突出的病性特点。3.海洛因成瘾者在吸毒年限为4-12年,年龄在20-39岁间,其端粒长度变化与其年龄有一定的关系,即端粒长度可随年龄增大而缩短,且与肝、脾、肾、气滞、湿、痰、气虚、阳虚及血虚等证素的形成有关。4.冰毒成瘾者端粒长度的缩短主要与中医肝、湿、热证素的形成有关。
[Abstract]:Aim: to explore the relationship between telomere length of peripheral blood leukocytes and TCM syndromes in heroin addicts and meth addicts, so as to explore the possible internal mechanism of the effects of heroin and meth on human health at gene level. Methods: all subjects were Han nationality aged 20 to 49. There were 342 drug addicts in the experimental group, including 206 heroin addicts (M 129 and F 77) and 136 meth users (M 80 and F 56), and the experimental group consisted of 342 drug addicts, including 206 heroin addicts (M 129 and F 77) and 136 meth addicts (M 80 and F 56). All of them were voluntary drug abusers who participated in the investigation from 2012 to 2014 in Fuzhou Public Security compulsory Drug rehab Center and the compulsory Drug Addiction Administration Bureau of the Department of Justice. In the control group, 200 healthy subjects (M 115 and F 85) were recruited from the physical examination Center of the second people's Hospital of Fujian Province. The characteristics of TCM syndrome elements were extracted by syndrome differentiation method, and the telomere length of peripheral blood leukocytes was measured by QPCR basic technique. Results: 1. The age of healthy group, heroin group and meth group were all 20 to 49 years old, and there was no difference in age and sex distribution among the three groups (P0.05). The telomere length of leukocytes in all participants was negatively correlated with age and sex (P0.05). After adjusting for age and sex, the telomere length of heroin group and meth group was shorter than that of healthy group (P0.05). 2. The telomere length of heroin group and meth group was shorter than that of healthy group (P0.05). After adjusting for age and sex, there was no significant difference in telomere length between heroin group and meth group (P0.05), and there was no significant correlation between telomere length and drug use duration between the two groups (P0.05). There was no significant difference in telomere length between the two groups (P0.05). The distribution of TCM syndrome elements in heroin and meth addicts was roughly the same, with liver, spleen and kidney as the main diseases, especially the liver; Qi stagnation was the most common syndrome in disease; Qi deficiency was the most common in deficiency syndrome. 4. After adjusting for age and sex, the relationship between the changes of telomere length and TCM syndromes was observed. There was no significant difference in telomere length between heroin group and healthy group (P0.05). There was a significant difference between the telomere length of the ice group and the healthy group (P0.05), and the telomere length of the meth group was shorter than that of the healthy group (P0.05), and the telomere length of the ice group was shorter than that of the healthy group (P0.05). In heroin group, the telomere length of 20-29-year-old group was significantly different from that of 30-39-year-old group (P0.01), and the telomere length of 30-29-year-old group was shorter than that of 20-29-year-old age group. The telomere length of liver, spleen, kidney, qi stagnation, dampness, phlegm, qi deficiency, yang deficiency and blood deficiency were significantly different between two age groups (P0.05). Conclusion: 1. Heroin or meth can cause telomere damage in peripheral blood leukocytes of drug addicts and shorten telomere length. 2. Heroin or meth can cause dysfunction of Zang-fu organs and injury of qi-blood fluid, especially in liver, kidney and spleen, especially in liver. Stagnation of qi and deficiency of qi are the most prominent disease characteristics of heroin and meth addicts. 3. Heroin addicts take drugs for 4-12 years, aged between 20 and 39, and their telomere length changes have a certain relationship with their age, that is, telomere length can be shortened with age, and with liver, spleen, kidney, qi stagnation, dampness, phlegm, qi deficiency, Yang deficiency and blood deficiency related to the formation of syndromes. 4. The shortening of telomere length in meth addicts is mainly related to the formation of liver, dampness and pyrogen in traditional Chinese medicine.
【学位授予单位】：福建中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R277.7

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