不同波形电针预处理对大鼠脓毒症脑损伤的影响

发布时间：2019-05-06 13:50

【摘要】：目的观察不同波形电针预处理对大鼠脓毒症脑损伤的影响。方法成年雄性SD大鼠80只,3月龄,体重200~220g,随机分为五组:假手术组(Sham组)、盲肠结扎穿孔组(CLP组)、连续波组(CW组)、疏密波组(DW组)及间断波组(IW组),每组16只。采用盲肠结扎穿孔(CLP)术制备脓毒症模型。观察大鼠脑水肿、额叶皮质病理结构(HE染色)、凋亡(TUNEL染色)及TLR4表达变化(免疫组织化学染色);测定血清、额叶皮质肿瘤坏死因子(TNF)-α、白细胞介素(IL)-6、丙二醛(MDA)浓度和超氧化物歧化酶(SOD)活性。结果与Sham组比较,CLP组、CW组、DW组及IW组出现脑水肿、脑损伤及神经元凋亡明显加重(P0.05);血清及额叶皮质TNF-α、IL-6及MDA浓度明显升高,SOD活性明显降低(P0.05);TLR4表达明显增加(P0.05)。与CLP组比较,CW组、DW组及IW组脑水肿、脑损伤及神经元凋亡明显减轻(P0.05);血清及额叶皮质TNF-α、IL-6及MDA浓度明显下降,SOD活性明显升高(P0.05);TLR4表达量明显降低(P0.05)。电针组之间比较,DW组改善作用最明显,IW组次之,CW组较差(P0.05)。结论不同波形电针能减轻大鼠脓毒症脑损伤且疏密波最佳,可能与抑制炎症、氧化应激及神经元凋亡有关。
[Abstract]:Objective to observe the effects of electroacupuncture preconditioning with different waveforms on brain injury in rats with sepsis. Methods 80 adult male SD rats were randomly divided into five groups: sham operation group (Sham group), cecal ligation and puncture group (CLP group), continuous wave group (CW group), dense wave group (DW group) and intermittent wave group (IW group). There are 16 rats in each group. Sepsis model was established by cecal ligation and puncture (CLP). The changes of brain edema, pathological structure of frontal cortex (HE staining), apoptosis (TUNEL staining) and TLR4 expression (immunohistochemical staining) were observed. Serum and frontal cortex tumor necrosis factor-伪 (TNF)-伪), interleukin-6 (IL-6), malondialdehyde (MDA) concentration and superoxide dismutase (SOD) (SOD) activity were measured. Results compared with Sham group, brain edema, brain injury and neuron apoptosis were significantly increased in CLP group, CW group, DW group and IW group (P0.05). The concentrations of TNF- 伪, IL-6 and MDA in serum and frontal cortex were significantly increased, while the activity of SOD was significantly decreased (P0.05), while the expression of TLR4 was significantly increased (P0.05). Compared with CLP group, brain edema, brain injury and neuron apoptosis were significantly reduced in CW group, DW group and IW group (P0.05), TNF- 伪, IL-6 and MDA concentrations in serum and frontal cortex were significantly decreased and SOD activity was significantly increased (P0.05). The expression of TLR4 decreased significantly (P 0.05). Compared with the electro-acupuncture group, the improvement effect of DW group was the most obvious, followed by IW group, and CW group was worse (P0.05). Conclusion electroacupuncture with different waveforms can alleviate the brain injury in rats with sepsis, which may be related to the inhibition of inflammation, oxidative stress and neuronal apoptosis.
【作者单位】：西南医科大学附属医院中医科;西南医科大学附属医院麻醉科;
【基金】：国家自然科学基金(81301055) 西南医科大学科研课题(20130422)
【分类号】：R245

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