肺抑瘤膏治疗气虚体质非小细胞肺癌的实验研究与临床观察

发布时间：2019-05-08 18:25

【摘要】：目的:基于中医体质学说探讨肺抑瘤膏治疗气虚质非小细胞肺癌的疗效及作用机理。方法:1.动物实验部分。用烟熏法建立气虚质小鼠模型,造模成功后向气虚质小鼠接种Lewis肺腺癌细胞建立病症结合模型。接种7天后,将造模成功小鼠随机分为模型对照组、肺抑瘤膏组、吉非替尼组、肺抑瘤膏+吉非替尼组,分别进行药物干预,对比各组小鼠体重变化、力竭游泳时间、免疫指标、瘤重及VEGF表达情况。2.临床观察部分。将64名符合条件的气虚体质中晚期非小细胞肺癌患者随机分为中药加化疗组(34例)和单纯化疗组(30例),对比两组患者的近期疗效、无进展生存期、卡氏评分、体重、中医临床症状及药物毒副反应情况。结果:1.动物实验结果。气虚体质Lewis肺腺癌小鼠动物模型造模成功,肺抑瘤膏组在小鼠体重变化、力竭游泳时间、免疫指标、瘤重及VEGF表达方面与模型对照组均存在差异,与吉非替尼联合使用较单一用药效果更佳。2.临床观察结果。中药加化疗组在近期疗效、体重改变、KPS评分、中医临床症状积分、药物毒副反应方面均优于单纯化疗组。结论:1.使用烟熏法建立的气虚质小鼠模型在一般情况、免疫指标方面均异于对照组,该体质造模方法可行有效。2.肺抑瘤膏可有效改善中晚期非小细胞肺癌患者的近期疗效,减少临床症状,提高生活质量。3.肺抑瘤膏可纠正体质偏颇,改善气虚质患者免疫力低下、易疲乏的特点。4.肺抑瘤膏与靶向药物或化疗药物联合应用可起到减毒增效的作用。5.通过抑制VEDF表达来降低肿瘤血管生成,可能是肺抑瘤膏抑制肿瘤生长的作用机制之一。
[Abstract]:Objective: to explore the therapeutic effect and mechanism of lung-suppressive ointment on Qi-deficiency non-small cell lung cancer (NSCLC) based on physique theory of traditional Chinese medicine (TCM). Methods: 1. Animal experiment part. The model of qi deficiency mice was established by fumigation, and the disease binding model was established by inoculating Lewis lung gland cancer cells into qi deficiency mice. Seven days after inoculation, the successful mice were randomly divided into three groups: the model control group, the lung tumor suppression paste group, the gefitinib group and the lung tumor suppression ointment group. The mice in each group were treated with drugs, and the weight changes and swimming time of each group were compared. Immune index, tumor weight and VEGF expression. 2. Clinical observation part. Sixty-four patients with advanced non-small cell lung cancer in Qi deficiency constitution were randomly divided into two groups: Chinese medicine plus chemotherapy group (34 cases) and simple chemotherapy group (30 cases). The short-term curative effect, progression-free survival time, Karnaud score and body weight were compared between the two groups. Clinical symptoms and side effects of traditional Chinese medicine. Results: 1. The results of animal experiments. The animal model of lung cancer mice with qi deficiency constitution Lewis was successfully established. There were differences in body weight, exhaustive swimming time, immune index, tumor weight and VEGF expression between the lung cancer ointment group and the model control group. Combined with gefitinib is better than a single drug. 2. Clinical observation results. The traditional Chinese medicine plus chemotherapy group was superior to the simple chemotherapy group in short-term curative effect, weight change, KPS score, TCM clinical symptom score and drug toxicity. Conclusion: 1. The model of qi deficiency and quality mice established by fumigation method is different from that of the control group in general and immune indexes, and the physique modeling method is feasible and effective. 2. Lung anti-tumor ointment can effectively improve the short-term efficacy of patients with advanced non-small cell lung cancer, reduce clinical symptoms, and improve the quality of life. 3. Lung anti-tumor ointment can correct physical bias, improve the low immunity of patients with Qi deficiency, easy to fatigue characteristics. 4. Lung tumor suppressant ointment combined with targeted drugs or chemotherapy drugs can reduce toxicity and enhance the efficacy. 5. Down-regulation of tumor angiogenesis by inhibiting the expression of VEDF may be one of the mechanisms by which lung tumor suppressor ointment inhibits tumor growth.
【学位授予单位】：山东中医药大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R273

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