电针对颅脑损伤大鼠脑组织中AQP-4表达的影响

发布时间：2019-05-24 04:26

【摘要】：目的:对大鼠颅脑损伤后,于不同时间点开始介入电针治疗,观察其脑组织中AQP-4的表达和右侧前后肢回缩力变化与电针介入时间的关系,探索电针治疗颅脑损伤的最佳时间窗。方法:选用70只SD大鼠,随机分为空白组、模型对照组、电针治疗组(电针1组,电针2组),使用e CCI仪器在大鼠颅脑左侧开颅打击制备TBI(traumatic brain injury)模型。取大鼠"百会、关元",右侧前肢"曲池、合谷",右侧后肢"足三里、涌泉",电针1组于造模后4 h介入电针治疗,电针2组于第8天介入电针治疗,每组治疗各7 d。(1)造模后8 d、15 d和22 d分别测试并评价大鼠右侧前后肢肢体回缩力。(2)造模后8 d和15 d取大鼠创伤脑组织,一部分进行HE染色及免疫荧光染色,观察脑组织形态结构及脑组织中AQP-4蛋白的表达;一部分采用实时荧光定量PCR技术检测AQP-4mRNA的表达。结果:造模后8 d、15 d和22 d三个时间点电针1组前后肢肢体回缩力比电针2组、模型组恢复的好,比空白组稍差。光镜下,空白组脑组织形态结构正常;电针治疗组比模型组好,电针1组比电针2组好。脑组织中AQP-4蛋白的含量以及mRNA的表达,空白组、模型组、电针治疗组各组比较均有统计学差异(P0.01),其中电针1组升高最明显。结论:电针治疗TBI早期介入效果更佳,其机制可能是通过提高脑组织中AQP-4的表达,减轻脑水肿,达到保护神经元的作用。
[Abstract]:Objective: to observe the relationship between the expression of AQP-4 in brain tissue and the contractile force of right anterior and posterior limbs and the intervention time of EA at different time points after craniocerebral injury in rats. To explore the best time window for the treatment of craniocerebral injury by electro-acupuncture. Methods: 70 SD rats were randomly divided into blank group, model control group, EA treatment group (EA 1 group, EA 2 group). The TBI (traumatic brain injury) model was established by e CCI instrument in the left craniocerebral craniotomy of rats. The rats were treated with "Baihui, Guanyuan", "Quchi, Hegu" on the right forelimb and "Zusanli, Yongquan" on the right hindlimb. The rats in group 1 were treated with EA 4 hours after modeling, and the two groups were treated with EA on the 8th day. The contractility of the right anterior and posterior limbs of the rats was measured and evaluated on the 8th day, 15th and 22nd day after the model was established. (2) the traumatic brain tissue of the rats was taken 8 and 15 days after the establishment of the model, and the contractile force of the right anterior and posterior limbs was measured and evaluated at the 8th and 15th day after the establishment of the model. In part, HE staining and immunofluorescence staining were used to observe the morphology of brain tissue and the expression of AQP-4 protein in brain tissue. In part, the expression of AQP-4mRNA was detected by real-time fluorescence quantitative PCR. Results: on the 8th day, 15 d and 22 d after modeling, the contractile force of anterior and posterior limbs in group 1 was better than that in group 2, and the recovery in model group was slightly worse than that in blank group. Under light microscope, the morphology of brain tissue in the blank group was normal, the EA treatment group was better than the model group, and the EA 1 group was better than the EA 2 group. The content of AQP-4 protein and the expression of mRNA in brain tissue were significantly different in blank group, model group and electro-acupuncture treatment group (P 0.01), and the increase was most obvious in group 1. Conclusion: the effect of early intervention in the treatment of TBI is better, and the mechanism may be to increase the expression of AQP-4 in brain tissue, reduce brain edema and protect neurons.
【作者单位】：成都中医药大学针灸推拿学院;陕西中医药大学针灸推拿学院;
【基金】：国家自然科学基金面上项目(81674088) 陕西省自然科学基础研究计划项目(2015JZ025)
【分类号】：R245

【相似文献】