壮药篱栏网质量标准研究

发布时间：2019-05-28 03:18

【摘要】：目的:对壮药篱栏网进行真伪、优劣评价,并初步建立壮药篱栏网药材质量标准。方法:(1)通过基源鉴定、性状鉴定、显微鉴定与理化鉴定系统地对篱栏网进行生药学定性研究。(2)照《中华人民共和国药典》2015年版四部中规定的方法对篱栏网药材进行限量检查,包括水分、总灰分、酸不溶性灰分、浸出物等。(3)按照中药化学预实验方法对3个产地篱栏网进行成分预试。(4)以芦丁为对照品,采用紫外-可见光分光光度法对广西12个批次篱栏网药材进行总黄酮含量测定,确定其所含总黄酮的限量。(5)以绿原酸为对照品,采用高效液相色谱法对广西12个批次篱栏网药材进行绿原酸含量测定,以确定其所含绿原酸的限量。(6)基于抗菌效价的生物检定法检测篱栏网药材的生物活性。结果:(1)基源、性状、显微及薄层色谱鉴别均具显著性特征,可作为鉴别篱栏网药材真伪的依据。(2)实验结果表明,篱栏网药材水分含量范围为6.26%~9.96%,初步拟定其水分不得超过11.00%;总灰分含量范围为6.31~11.55%,初步拟定总灰分不得超过13.00%;酸不溶性灰分含量范围为0.20%~1.60%,初步拟定酸不溶性灰分不得超过1.70%;浸出物含量在4.69%~9.22%之间,初步拟定醇溶性浸出物含量不得少于4.00%。(3)化学成分预实验显示篱栏网中含有黄酮类、糖、多糖、苷类、生物碱、蒽醌类、蛋白质、氨基酸、有机酸及鞣质或酚类等化学成分类型。(4)广西12批次的篱栏网药材总黄酮含量测得值在5.13%~14.32%之间,初步拟定其所含总黄酮不得少于4.00%。(5)广西12批次篱栏网药材中绿原酸含量测得值在0.22%~0.64%之间,初步拟定其所含绿原酸(C16H18O9)不得少于0.20%。(6)篱栏网药材的对数剂量和抑菌效应呈线性关系,相关性好,基于抗菌效价的生物检定法可行,初步拟定篱栏网(0.5g·ml-1)的效价不得低于0.8000U/ml。结论:为篱栏网药材的准确鉴定和质量标准的制定提供了科学依据,也为篱栏网的开发应用研究奠定了基础。
[Abstract]:Objective: to evaluate the authenticity, merits and demerits of Zhuang medicine fence net, and to establish the quality standard of Zhuang medicine fence net. Methods: (1) through base source identification, character identification, The pharmacognosy of hedgerow net was systematically studied by microscopic identification and physical and chemical identification. (2) according to the methods stipulated in the 2015 edition of the Pharmacopoeia of the people's Republic of China, the limited amount of medicinal materials in hedgerow net, including moisture and total ash, was examined. Acid insoluble ash, extract, etc. (3) according to the chemical pre-experimental method of traditional Chinese medicine, the composition of hedgerow net from three producing areas was pre-tested. (4) rutin was used as reference material. The content of total flavonoids in 12 batches of hedgerow in Guangxi was determined by UV-vis spectrophotometry, and the limit of total flavonoids was determined. (5) chlorogenic acid was used as reference material. The content of chlorogenic acid in 12 batches of hedgerow net in Guangxi was determined by high performance liquid chromatography (HPLC) to determine the limit of chlorogenic acid. (6) the biological activity of hedgerow net was determined by bioassay based on antibacterial titer. Results: (1) the identification of base source, character, microscope and thin layer chromatography was significant, which could be used as the basis for distinguishing the authenticity of hedgerow net. (2) the experimental results showed that the water content of hedgerow net was 6.26% and 9.96%, The moisture content of the preliminary formulation shall not exceed 11.00%; The range of total ash content is 6.31 鈮，

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