四种“蒂达”类藏药提取物对四氯化碳损伤人肝细胞的保护作用研究

发布时间：2019-06-13 16:08

【摘要】：目的考察四种"蒂达"类藏药提取物对体外四氯化碳损伤人肝细胞的保护作用,为该类药材的开发利用提供药理学研究依据。方法采用四氯化碳诱导的人肝L-02细胞损伤模型,以四种"蒂达"类藏药提取物进行干预,水飞蓟宾为对照,MTT法测定细胞存活率并测定细胞培养上清液中的谷丙转氨酶(ALT)、谷草转氨酶(AST)的活力、丙二醛(MDA)的含量,以及细胞裂解上清液中的超氧化物气化酶(SOD)的活力。结果四种"蒂达"类藏药提取物均可显著提高肝细胞的存活率,可显著性降低四氯化碳致肝细胞损伤的转氨酶(ALT、AST)的活力,显著升高超氧化物气化酶(SOD)的活力,降低丙二醛(MDA)的含量,其中篦齿虎耳草提取物对上述指标的影响改变尤为显著。结论四种"蒂达"类藏药均可减轻四氯化碳致肝细胞损伤程度,其中篦齿虎耳草效果最为明显,其作用机制与抗自由基而减轻脂质过氧化损伤相关。
[Abstract]:Objective to investigate the protective effect of four kinds of Tibetan drug extract on human hepatocytes damaged by carbon tetrachloride in vitro, and to provide pharmacological research basis for the development and utilization of this kind of medicinal materials. Methods the injury model of human liver L 眉 02 cells induced by carbon tetrachloride was used to intervene with four kinds of "Tida" Tibetan drug extract. Silybin was used as control. The cell survival rate was measured by MTT assay, the activity of glutamic-pyruvic transaminase (ALT), glutamic oxaloacetic transaminase (AST), the content of malondialdehyde (MDA) in the cell culture medium, and the activity of superoxide gas enzyme (SOD) in the cell lytic medium. Results all the four kinds of Tibetan drug extract could significantly increase the survival rate of hepatocytes, significantly decrease the activity of transaminase (ALT,AST), the activity of (SOD) and the content of malondialdehyde (MDA) in hepatocytes damaged by carbon tetrachloride. The effect of Scutellaria grate extract on the above indexes was particularly significant. Conclusion all four kinds of "Dida" Tibetan drugs can reduce the degree of liver cell injury induced by carbon tetrachloride, among which Saxifraga grate has the most obvious effect, and its mechanism is related to anti-free radical and lipid peroxide damage.
【作者单位】：江西中医药大学中药资源与民族药研究中心;江西民族传统药现代科技与产业发展协同创新中心;江西中医药大学实验动物科技中心;
【基金】：国家自然科学基金(No.81360675) 国家科技重大专项子课题(No.2012ZX09103201-050)
【分类号】：R29

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