藏药喜马拉雅紫茉莉及陆额的质量评价初探
发布时间：2019-06-18 18:24
【摘要】：本研究通过现代技术手段对藏药材喜马拉雅紫茉莉及陆额进行初步的质量评价研究,为两种药材合理利用及进一步的开发提供理论依据。具体包括以下几个方面:1.HPLC法测定不同海拔、不同直径藏药喜马拉雅紫茉莉根中葫芦巴碱的含量方法:NH2色谱柱,流动相乙腈-水(87:13),检测波长264 nm。结果:线性范围1.0109~101.9μg/mL(r=0.9997)。平均回收率95.7%(n=9,RSD=1.12%)。结果:低海拔(2900 m)药材中葫芦巴碱含量171.5 mg/kg,高海拔3630 m以上7组药材平均含量267.3 mg/kg;某地药材四种不同直径(1.5~7.5 cm),葫芦巴碱含量依次为309.2、319.8、173.8、197.9 mg/kg。另外,某地直径3.5~4 cm和5.5~7 cm的药材葫芦巴碱含量相当,分别为296.11、305.13mg/kg。结论:推测喜马拉雅紫茉莉药材中葫芦巴碱的含量与海拔、药材生长年限有关:海拔较高的药材含量较高。药材生长初期,葫芦巴碱含量有上升趋势,到一定年限后,葫芦巴碱含量显著下降。2.喜马拉雅紫茉莉红外指纹图谱研究方法:制备9批药材的溴化钾压片,通过红外光谱仪检测并采集谱图,对比9批药材的红外光谱,应用红外分析软件(IR solution 1.3)计算相似值,并对相似度值进行聚类分析。结果:中华紫茉莉与喜马拉雅紫茉莉的红外光谱在667.3cm-1处吸收峰有明显区别,能准确地区分二者;相似度结果显示喜马拉雅紫茉莉样品间相似度均大于0.92,而与中华紫茉莉相似度均不超过0.85;对相似度值进行聚类分析结果提示喜马拉雅紫茉莉药材直径越相近,其红外光谱图更相似。结论:红外指纹图谱可作为鉴别喜马拉雅紫茉莉与中华紫茉莉的手段。3.HPLC法测定藏药材陆额中南天竹碱的含量色谱方法:C18色谱柱,流动相:ACN(A),20%ACN+0.1%(v/v)磷酸+0.167%(m/v)SDS(B);梯度洗脱(0~20 min,26%A:20~50min,26%→30%A),检测波长220 nm。结果:线性范围1.200~0.0096 mg/mL(r=0.9995)平均回收率105.8%。本实验对流动相中SDS加入量进行考察。结论:该方法准确、稳定,可用于检测陆额中南天竹碱的含量。4.陆额的化学成分研究方法:采用系统分离法对陆额的正丁醇部位进行分离,通过波普数据鉴定结构。结果:从陆额的正丁醇部位中分离鉴定了4个成分:6,7亚甲二氧基-2-甲基-1,2,3,4四氢异喹啉(6,7-Methylenedioxy-2-methyl-1,2,3,4-tetrahydroisoquinoline),前荷叶碱N-氧化物(pronuciferine N-oxide),Papraine,原阿片碱(Protopine)。首次为该植物中分离得到。
[Abstract]:In this study, the preliminary quality evaluation of the Himalayan purple jasmine and the land amount of the Tibetan medicinal materials was carried out by means of modern technical means, and the theoretical basis for the rational utilization and further development of the two kinds of medicinal materials was provided. The method comprises the following aspects:1. the method for determining the content of the cucurbita in the Himalayan purple jasmine root with different altitude and different diameters is determined by the HPLC method, the NH2 chromatographic column, the mobile phase acetonitrile-water (87:13) and the detection wavelength of 264nm are detected. Results: The linear range was 1.0109 ~ 101.9 & mu; g/ mL (r = 0.9997). The average recovery was 95.7% (n = 9, RSD = 1.12%). Results: The average content of fenugreek base in the low altitude (2900m) medicinal materials was 171.5 mg/ kg, the average content was 267.3 mg/ kg in 7 groups with high altitude of 3630 m, and the content of the four different diameters (1.5 to 7.5 cm) of the medicinal materials was 309.2, 319.8, 173.8 and 197.9 mg/ kg. In addition, the content of cucurbitacin in the medicinal materials with a diameter of 3.5 to 4 cm and 5.5 to 7 cm was comparable, which was 296.11 and 305.13 mg/ kg, respectively. Conclusion: The content of fenugreek base in the Himalayan purple jasmine is related to the altitude and the growth period of the medicinal materials: the higher the content of the medicinal materials with higher elevation. In that early stage of the growth of the medicinal material, the content of the cucurbitacin was increase, and after a certain period of time, the content of the fenugreek base decreased significantly. The research method of the infrared fingerprint of the Himalayan purple jasmine is as follows:9 batches of potassium bromide tablets are prepared, and the spectrum images are detected and collected by the infrared spectrometer, the infrared spectrum of the nine batches of medicinal materials is compared, the similar values are calculated by using the infrared analysis software (IR solution 1.3), and the similarity values are clustered. The results show that the infrared spectra of the Chinese violet jasmine and the Himalayan purple jasmine are obviously different from the absorption peak at 667.3 cm-1, and can be divided into two regions; the similarity results show that the similarity between the samples of the Himalayan purple jasmine samples is more than 0.92, and the similarity with the Chinese violet jasmine is not more than 0.85; The results of cluster analysis on the similarity value indicate that the diameter of the Himalayan purple jasmine is similar, and the infrared spectrum is more similar. Conclusion: The infrared fingerprint can be used as a means of identifying the Himalayan purple jasmine and the Chinese violet jasmine.3. The method of HPLC method for the determination of the content of the nanophanine in Tibetan medicinal materials: C18 column, mobile phase: ACN (A),20% ACN + 0.1% (v/ v) phosphoric acid + 0.167% (m/ v) SDS (B), gradient elution (0-20 min,26% A:20-50 min,26% and 30% A), The detection wavelength is 220 nm. Results: The average recovery of linear range of 1.200 ~ 0.0096mg/ mL (r = 0.9995) was 105.8%. The amount of SDS in the mobile phase was investigated in this experiment. Conclusion: The method is accurate and stable, and can be used to detect the content of the bamboo base in the land. The chemical composition of the land was studied by using the system separation method to separate the n-butanol part of the land, and the structure was identified by the Pop data. Results: Four components were identified from the n-butanol fraction of the land,6,7-methylenedioxy-2-methyl-1,2,3,4-dioxy-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2 For the first time in the plant.
【学位授予单位】：西藏大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R29
[Abstract]:In this study, the preliminary quality evaluation of the Himalayan purple jasmine and the land amount of the Tibetan medicinal materials was carried out by means of modern technical means, and the theoretical basis for the rational utilization and further development of the two kinds of medicinal materials was provided. The method comprises the following aspects:1. the method for determining the content of the cucurbita in the Himalayan purple jasmine root with different altitude and different diameters is determined by the HPLC method, the NH2 chromatographic column, the mobile phase acetonitrile-water (87:13) and the detection wavelength of 264nm are detected. Results: The linear range was 1.0109 ~ 101.9 & mu; g/ mL (r = 0.9997). The average recovery was 95.7% (n = 9, RSD = 1.12%). Results: The average content of fenugreek base in the low altitude (2900m) medicinal materials was 171.5 mg/ kg, the average content was 267.3 mg/ kg in 7 groups with high altitude of 3630 m, and the content of the four different diameters (1.5 to 7.5 cm) of the medicinal materials was 309.2, 319.8, 173.8 and 197.9 mg/ kg. In addition, the content of cucurbitacin in the medicinal materials with a diameter of 3.5 to 4 cm and 5.5 to 7 cm was comparable, which was 296.11 and 305.13 mg/ kg, respectively. Conclusion: The content of fenugreek base in the Himalayan purple jasmine is related to the altitude and the growth period of the medicinal materials: the higher the content of the medicinal materials with higher elevation. In that early stage of the growth of the medicinal material, the content of the cucurbitacin was increase, and after a certain period of time, the content of the fenugreek base decreased significantly. The research method of the infrared fingerprint of the Himalayan purple jasmine is as follows:9 batches of potassium bromide tablets are prepared, and the spectrum images are detected and collected by the infrared spectrometer, the infrared spectrum of the nine batches of medicinal materials is compared, the similar values are calculated by using the infrared analysis software (IR solution 1.3), and the similarity values are clustered. The results show that the infrared spectra of the Chinese violet jasmine and the Himalayan purple jasmine are obviously different from the absorption peak at 667.3 cm-1, and can be divided into two regions; the similarity results show that the similarity between the samples of the Himalayan purple jasmine samples is more than 0.92, and the similarity with the Chinese violet jasmine is not more than 0.85; The results of cluster analysis on the similarity value indicate that the diameter of the Himalayan purple jasmine is similar, and the infrared spectrum is more similar. Conclusion: The infrared fingerprint can be used as a means of identifying the Himalayan purple jasmine and the Chinese violet jasmine.3. The method of HPLC method for the determination of the content of the nanophanine in Tibetan medicinal materials: C18 column, mobile phase: ACN (A),20% ACN + 0.1% (v/ v) phosphoric acid + 0.167% (m/ v) SDS (B), gradient elution (0-20 min,26% A:20-50 min,26% and 30% A), The detection wavelength is 220 nm. Results: The average recovery of linear range of 1.200 ~ 0.0096mg/ mL (r = 0.9995) was 105.8%. The amount of SDS in the mobile phase was investigated in this experiment. Conclusion: The method is accurate and stable, and can be used to detect the content of the bamboo base in the land. The chemical composition of the land was studied by using the system separation method to separate the n-butanol part of the land, and the structure was identified by the Pop data. Results: Four components were identified from the n-butanol fraction of the land,6,7-methylenedioxy-2-methyl-1,2,3,4-dioxy-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2-methyl-1,2,3,4-tetrahydro-2 For the first time in the plant.
【学位授予单位】：西藏大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R29
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