香延止痛方对寒凝血瘀证原发性痛经大鼠的治疗效果及其作用机制
发布时间：2019-07-01 19:36
【摘要】：目的探讨香延止痛方对寒凝血瘀证原发性痛经大鼠的治疗效果及其作用机制。方法选择大鼠50只,随机分为空白组、模型组、西药组及中药低、高剂量组,模型组、西药组及中药低、高剂量组建立寒凝血瘀证原发性痛经模型,造模第7天,空白组和模型组予蒸馏水2 mL/只灌胃,西药组予布洛芬按每日0.002 g灌胃2 mL/只,中药低剂量组给予香延止痛方水煎剂2 mL/只(含生药2.38 g),中药高剂量组给予香延止痛方水煎剂2 mL/只(含生药11.9 g)。各组均连续灌胃6天。末次灌胃30 min,除空白组外,其余各组腹腔注射缩宫素,观察大鼠扭体潜伏时间及30 min内扭体反应次数。采用ELISA法检测各组子宫组织上清液INF-γ、IL-4水平,RT-PCR法检测子宫组织上清液INF-γ、IL-4 mRNA表达。结果中药低、高剂量组30 min内扭体次数明显少于模型组(P均0.05),扭体潜伏时间有延长趋势(P均0.05)。与空白组比较,模型组子宫组织上清液IFN-γ水平降低、IL-4水平升高,IFN-γ/IL-4降低,TNF-αmRNA表达降低,IL-4 mRNA表达升高(P均0.05);与模型组比较,中药低、高剂量组IFN-γ含量升高,IL-4含量降低,IFN-γ/IL-4升高,TNF-αmRNA表达升高,IL-4 mRNA表达降低(P均0.05);但中药低、高剂量组比较差异无统计学意义(P均0.05)。结论寒凝血瘀型原发性痛经大鼠存在Th1/Th2平衡漂移,香延止痛方可能通过调节Th1/Th2的平衡状态缓解痛经症状。
[Abstract]:Objective to investigate the therapeutic effect and mechanism of Xiangyan Zhitong recipe on primary dysmenorrhea rats with cold coagulation and blood stasis syndrome. Methods Fifty rats were randomly divided into blank group, model group, western medicine group and low traditional Chinese medicine group. The model of primary dysmenorrhea with cold coagulation and blood stasis syndrome was established in the high dose group. On the 7th day, the blank group and the model group were given distilled water 2 mL/ only intragastrically, and the western medicine group was given ibuprofen 0.002 g per day for 2 mL/. The low dose group of traditional Chinese medicine was given 2 mL/ decoction of Xiangyan Zhitong recipe (containing 2.38 g), of traditional Chinese medicine). The high dose group of traditional Chinese medicine was given 2 mL/ decoction of Xiangyan Zhitong recipe (including 11.9 g). Of crude drug). Each group was given intragastrically for 6 days. Except the blank group, oxytocin was injected intraperitoneally for the last time for 30 min,. The latent time of writhing and the number of writhing reaction in 30 min were observed. The levels of INF- 纬 and IL-4 in the culture medium of each group were detected by ELISA, and the expression of INF- 纬 and IL-4 mRNA in the culture medium of uterine tissue was detected by RT-PCR. Results the number of writhing within 30 min in the high dose group was significantly lower than that in the model group (P 0.05), and the latent time of writhing was prolonged (all P 0.05). Compared with the blank group, the level of IFN- 纬, the level of IL-4, the ratio of IFN- to IL-4, the expression of TNF- 伪 mRNA and the expression of IL-4 mRNA in the uterine tissue of the model group were decreased, and the contents of IFN- 纬, IL-4, IFN- 纬 / IL-4, TNF- 伪 mRNA and IL-4 mRNA in the model group were lower than those in the model group (P < 0.05), while those in the high dose group were significantly higher than those in the model group (P < 0.05), and the expression of TNF- 伪 mRNA and IL-4 mRNA in the model group were significantly higher than those in the model group (P < 0.05). However, there was no significant difference between the high dose group and the low dose group (P 0.05). Conclusion there is Th1/Th2 equilibrium drift in rats with primary dysmenorrhea of cold coagulation and stasis type. Xiangyan Zhitong recipe may relieve dysmenorrhea symptoms by regulating the balance of Th1/Th2.
【作者单位】： 山东中医药大学第一临床医学院;山东中医药大学附属医院;烟台市中医医院;山东省医学科学院基础医学研究所;
【基金】：山东省重点研发计划项目(2015GSF119010)
【分类号】：R271.113
本文编号：2508743
[Abstract]:Objective to investigate the therapeutic effect and mechanism of Xiangyan Zhitong recipe on primary dysmenorrhea rats with cold coagulation and blood stasis syndrome. Methods Fifty rats were randomly divided into blank group, model group, western medicine group and low traditional Chinese medicine group. The model of primary dysmenorrhea with cold coagulation and blood stasis syndrome was established in the high dose group. On the 7th day, the blank group and the model group were given distilled water 2 mL/ only intragastrically, and the western medicine group was given ibuprofen 0.002 g per day for 2 mL/. The low dose group of traditional Chinese medicine was given 2 mL/ decoction of Xiangyan Zhitong recipe (containing 2.38 g), of traditional Chinese medicine). The high dose group of traditional Chinese medicine was given 2 mL/ decoction of Xiangyan Zhitong recipe (including 11.9 g). Of crude drug). Each group was given intragastrically for 6 days. Except the blank group, oxytocin was injected intraperitoneally for the last time for 30 min,. The latent time of writhing and the number of writhing reaction in 30 min were observed. The levels of INF- 纬 and IL-4 in the culture medium of each group were detected by ELISA, and the expression of INF- 纬 and IL-4 mRNA in the culture medium of uterine tissue was detected by RT-PCR. Results the number of writhing within 30 min in the high dose group was significantly lower than that in the model group (P 0.05), and the latent time of writhing was prolonged (all P 0.05). Compared with the blank group, the level of IFN- 纬, the level of IL-4, the ratio of IFN- to IL-4, the expression of TNF- 伪 mRNA and the expression of IL-4 mRNA in the uterine tissue of the model group were decreased, and the contents of IFN- 纬, IL-4, IFN- 纬 / IL-4, TNF- 伪 mRNA and IL-4 mRNA in the model group were lower than those in the model group (P < 0.05), while those in the high dose group were significantly higher than those in the model group (P < 0.05), and the expression of TNF- 伪 mRNA and IL-4 mRNA in the model group were significantly higher than those in the model group (P < 0.05). However, there was no significant difference between the high dose group and the low dose group (P 0.05). Conclusion there is Th1/Th2 equilibrium drift in rats with primary dysmenorrhea of cold coagulation and stasis type. Xiangyan Zhitong recipe may relieve dysmenorrhea symptoms by regulating the balance of Th1/Th2.
【作者单位】： 山东中医药大学第一临床医学院;山东中医药大学附属医院;烟台市中医医院;山东省医学科学院基础医学研究所;
【基金】：山东省重点研发计划项目(2015GSF119010)
【分类号】：R271.113
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