蒙药五味沙棘散对吸烟致小鼠肺部炎症的改善作用及机制研究

发布时间：2019-08-15 18:28

【摘要】：目的:研究蒙药五味沙棘散(WSP)对吸烟所致小鼠肺部炎症的改善作用及其机制。方法:将30只ICR小鼠随机分为空白组(生理盐水)、模型组(生理盐水)和WSP组(2 g/kg)。模型组和WSP组小鼠采用被动吸烟法复制肺部炎症损伤模型,并于造模的同时每天ig相应药物1次,连续28 d。给药结束后,采用酶联免疫吸附法检测小鼠肺泡灌洗液(BALF)中肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)、IL-6和IL-10水平;苏木精-伊红染色后光镜下观察小鼠肺组织病理变化;Western blot法检测小鼠肺组织中细胞外信号调节蛋白激酶1/2(ERK1/2)、磷酸化ERK1/2(p-ERK1/2)、p38丝裂原活化蛋白激酶(p38 MAPK)、磷酸化p38 MAPK(p-p38 MAPK)、核因子κB p65(NF-κB p65)和磷酸化NF-κB p65(p-NF-κB p65)蛋白表达。结果:与空白组比较,模型组小鼠BALF中TNF-α、IL-1β和IL-6水平明显升高(P0.01),肺组织发生明显炎症病变,肺组织中p-ERK1/2、p-p38 MAPK、pNF-κB p65蛋白表达水平明显升高(P0.01)。与模型组比较,WSP组大鼠BALF中TNF-α、IL-1β水平明显降低(P0.05),肺组织炎症损伤明显改善,肺组织中p-p38 MAPK和p-NF-κB p65蛋白表达水平明显降低(P0.05)。结论:WSP可能通过阻断p38MAPK、NF-κB p65蛋白的磷酸化来抑制炎症因子TNF-α和IL-6的高表达,从而发挥其对吸烟所致小鼠肺部炎症损伤的改善作用。
[Abstract]:Objective: to study the effect and mechanism of Mongolian medicine Wuwei Hippophae rhamnoides Powder (WSP) on pulmonary inflammation induced by smoking in mice. Methods: 30 ICR mice were randomly divided into blank group (saline), model group (saline) and WSP group (2 g/kg). The model group and WSP group were used to establish the model of pulmonary inflammatory injury by passive smoking, and the corresponding drugs of ig were made once a day for 28 days. After administration, the levels of tumor necrosis factor 伪 (TNF- 伪), IL 1 尾 (IL-1 尾), IL-6 and IL-10 in (BALF) of alveolar lavage fluid were detected by enzyme linked immunosorbent assay (Elisa), and the pathological changes of lung tissue were observed under light microscope after hematoxylin and eosin staining. The expression of extracellular signal-regulated protein kinase 1 鈮，

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