total saponins of Panax notoginseng nonalcoholic fatty liver
本文关键词:三七总皂苷改善高脂诱导脂肪肝大鼠模型氧化应激及胰岛素抵抗的研究,由笔耕文化传播整理发布。
三七总皂苷改善高脂诱导脂肪肝大鼠模型氧化应激及胰岛素抵抗的研究
Study on the Mechanism of Action of Total Saponins of Panax Notoginseng in Ameliorating Oxida- tive Stress and Insulin Resistance in Rats Fed with High Fat Diet
[1] [2] [3] [4] [5] [6] [7] [8]
ZHANG Sheng-sheng1, WU Zhen-yu1 ,CHEN Jian-ming2 ,GUO Qian-kun1 ,LI Lin1 ,WANG Zheng-fang1 ,GAO Yue3 ,and MA Zeng- chun3 1 Center for Digestive Disease, Beijing TCM Hospital
[1]首都医科大学附属北京中医医院消化中心,北京100010; [2]中国人民解放军二五一医院消化科,河北075000; [3]中国人民解放军军事医学科学院中药药理与毒理研究室,北京100850
文章摘要:目的观察三七总皂苷能否改善高脂诱导脂肪肝模型大鼠体内氧化应激及胰岛素抵抗(insulinresistance,IR)。方法50只SD大鼠平均分为正常组、模型组、三七总皂苷大剂量组[160mg/(kg·d)]、三七总皂苷小剂量组[80mg/(kg·d)]及双环醇组[100mg/(kg·d)],每组10只。正常组采用标准饲料喂养,模型组及给药组采用高脂饲料喂养。正常组和模型组给予蒸馏水灌胃,给药组灌胃给药4周。4周后测肥胖评定指数(Lee’S指数)、体重;测肝湿重;检测各组大鼠肝功能(ALT、AST);检测空腹血糖(fastingbloodglucose,FBG)、空腹胰岛素(fastinginsulin,FINS)水平,,计算胰岛素敏感指数(insulinsensitiveidex,ISI)及胰岛素抵抗指数(insulinresistanceindex,IRl);检测血清TNF.仅、丙二醛(malond.ialdehyde,MDA)、总超氧化物歧化酶(totalsuperoxidedisumutase,T.SOD)、羟自由基(.OH)及血清总抗氧化能力(totalantioxidantcapacity,T—AOC)水平。肝脏石蜡切片HE染色观察病理学改变。结果与正常组比较,模型组大鼠肝湿重、Lee’S指数均升高(P〈0.05),光镜下肝脏出现明显脂肪变性。与模型组比较,三七总皂苷及双环醇治疗可以明显改善肝湿重、Lee’S指数(P〈0.05,P〈0.01)及ALT、AST等指标(P〈0.05,P〈0.01);减轻肝细胞脂肪沉积。模型组大鼠血清.OH、MDA、TNF-α.较正常组均显著增高(P〈0.01),T—SOD及T-AOC活性降低(P〈0.01),且伴有IR。与模型组比较,经过三七总皂苷治疗后,-OH、MDA、TNF-α浓度降低(P〈0.05,P〈0.01),而T—SOD及T-AOC的活性恢复(P〈0.05,P〈0.01),IR也得到明显改善(P〈0.01)。结论三七总皂苷的抗氧化应急效应及改善IR效应是其对非酒精性脂肪肝疗效的部分机制。
Abstr:Objective To investigate whether total saponins ofPanax notoginseng (tPNS) can a- meliorate oxidative stress and insulin resistance (IR) in the high fat induced nonalcoholic fatty liver dis- ease (NAFLD) rat model and to explore the relationship between oxidative stress and IR. Methods To- tally 50 healthy rats were randomly divided into the normal control group ( NC), the model group, the high dose tPNS group (at the daily dose of 160 mg/kg), the low dose tPNS group (at the daily dose of 80 mg/kg), and the bicyclol group (at the daily dose of 100 mg/kg), 10 in each group. Rats in the NC group were fed with standard forage. Those in the rest group were fed with high fat forage. Distilled water was given by gastrogavage to those in the NC group and the model group. Corresponding medication was performed for 4 weeks. Four weeks later Lee's index and body weight were measured. All rats were sacri- ficed to detect the wet weight of livers. Their sera was isolated and detected to calculate liver functions (serum ALT and AST levels). Levels of fasting blood glucose (FBG) and fasting insulin (FINS) were de- tected. Insulin sensitive index (ISI) and insulin resistance index (IRI) were calculated. Serum levels of TNF-eL and malondialdehyde (MDA), contents of total superoxide dismutase (T-SOD), hydroxy radical level (-OH), and total antioxidant capacity (T-AOC) were measured. Pathological changes of livers was observed by HE staining of paraffin section. Results Compared with the NC group, rats' wet liver weight and Lee's index increased in the model group (P 〈0.05), and results of light microscopy showed that ob- vious fatty degeneration occurred in livers. Compared with the model group, rats' wet l
文章关键词:
Keyword::total saponins of Panax notoginseng nonalcoholic fatty liver disease oxidative stress insulin resistance protective mechanism
课题项目:北京市中医科技重点规划项目(No.KJTS2011_91);北京市卫生系统高层次卫生技术人才培养计划(No.211-2-13)
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本文关键词:三七总皂苷改善高脂诱导脂肪肝大鼠模型氧化应激及胰岛素抵抗的研究,由笔耕文化传播整理发布。
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