基质辅助激光解吸飞行时间质谱分析生物样品中甲基苯丙胺

发布时间：2018-02-02 23:12

本文关键词： MALDI-TOF-MS 碳纳米管甲基苯丙胺尿样头发　出处：《中国司法鉴定》2009年05期 　论文类型：期刊论文

【摘要】：目的建立尿样和头发中甲基苯丙胺的基质辅助激光解吸飞行时间质谱(matrix-assisted laser desorption/ionization time of flight mass spectrometry,MALDI-TOF-MS)分析方法。方法尿样采用液液提取,头发经0.1mol/L盐酸水解后采用液液提取,以碳纳米管为基质应用MALDI-TOF-MS法检测。结果尿样中甲基苯丙胺的最低检测限(LOD)为0.5μg/mL,线线范围为线性范围为0.5～100μg/mL(R2=0.9970);毛发中甲基苯丙胺的最低检测限(LOD)为0.4ng/mg,线性范围为0.4～60ng/mg(R2=0.9976),对送检案例中尿样和头发检材进行检测,效果良好。结论本方法适用于尿样和头发中甲基苯丙胺的分析,与传统气相色谱质谱联用和液相色谱-质谱联用相比,分析速度更快,适合大批量样品同时分析。
[Abstract]:Objective to establish a matrix assisted laser desorption time-of-flight mass spectrometry and urine methamphetamine in hair (matrix-assisted laser desorption/ionization time of flight mass spectrometry, MALDI-TOF-MS) analysis method. Methods urine samples by liquid-liquid extraction, the hair by 0.1mol/L salt after acid hydrolysis by liquid-liquid extraction, using carbon nanotubes as detection matrix using MALDI-TOF-MS method. Results the detection in urine methamphetamine limit (LOD) for 0.5 g/mL line range is linear in the range of 0.5 to 100 g/mL (R2=0.9970); the lowest detection limit of methamphetamine in hair (LOD) for 0.4ng/mg, the linear range of 0.4 ~ 60ng/mg (R2=0.9976), detection of urine and hair samples from cases with good results. Conclusion this method is suitable for analysis of urine and methamphetamine in hair, compared with the traditional gas chromatography combined with mass spectrometry and liquid chromatography mass spectrometry The analysis speed is faster and suitable for the simultaneous analysis of large quantities of samples.

【作者单位】：司法部司法鉴定科学技术研究所上海市法医学重点实验室;复旦大学上海医学院;
【基金】：上海市自然科学基金资助项目(06ZR14159)
【分类号】：D919.4
【正文快照】： 甲基苯丙胺(methamphetamine,MA)是一种舞厅等娱乐场所内滥用最严重的苯丙胺类兴奋剂,具有兴奋中枢神经和周围神经、抑制食欲、致幻等作用[1]。目前涉及MA的案件不断上升,进行尿样和头发分析的案件也越来越多,建立快速准确、适合大量样品同时分析的方法是毒物分析工作者面临

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