3个miniSTR基因座四色荧光复合分型体系的构建及应用

发布时间：2018-02-23 17:48

本文关键词： 法医物证学 miniSTR 复合扩增荧光标记降解检材　出处：《中国法医学杂志》2009年03期 　论文类型：期刊论文

【摘要】：目的建立D3S3053、D6S474、D20S482(扩增片段150bp)3个miniSTR基因座四色荧光复合分型体系,用于高度降解DNA样本的基因分型。方法采用6-FAM、HEX、TAMRA荧光染料标记D20S482、D3S3053、D6S474基因座上游引物,构建并优化复合扩增体系,在ABI 310遗传分析仪上对扩增产物进行电泳分析,Genemapper 3.2软件分析产物片段大小并进行分型。采用上述体系对120份河北汉族健康无关个体血样进行检测,并计算群体遗传学参数。比较该体系与ID试剂盒用于高度降解检材的成功率及灵敏度。结果D3S3053、D6S474、D20S482 3个miniSTR基因座荧光标记复合扩增分型体系稳定可靠,灵敏度达50pg,用于高度降解检材分析的成功率明显高于ID试剂盒。3个基因座在河北汉族人群中的累积个人识别能力为0.998,累积非父排除率为0.84。结论该系统可用于分析高度降解DNA样本的基因分型,进行法医学个人识别和亲权鉴定。
[Abstract]:Objective to establish a four-color fluorescent multiplex typing system of D3S3053-D6S474D20S482 (amplified fragment 150bp) for the genotyping of highly degraded DNA samples. Methods the upstream primers of D20S482D3S3053D6S474 locus were labeled with 6-FAMHE-HEXTA-TAMRA, and the compound amplification system was constructed and optimized. ABI 310 genetic analyzer was used to analyze the amplified products by electrophoretic analysis and genotyping using Genemapper 3.2 software. The above system was used to detect the blood samples of 120 healthy unrelated individuals in Hebei Han nationality. The population genetic parameters were calculated, and the success rate and sensitivity of the system and ID kit were compared. Results the multiplex amplification system of D3S3053, D6S474, D20S482 miniSTR locus was stable and reliable. The sensitivity was 50 PG, and the success rate of high degradation detection was significantly higher than that of ID kit. The cumulative personal identification ability of three loci in Hebei Han population was 0.998, and the cumulative non-paternal exclusion rate was 0.84.Conclusion this system can be used for analysis. Genotyping of highly degraded DNA samples, To conduct forensic personal identification and paternity identification.
【作者单位】：河北医科大学法医学系河北省法医学重点实验室;
【基金】：河北省“十一五”科技支撑计划项目(072461479D)
【分类号】：D919

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