GC法检测血液和尿液中甲基苯丙胺和咖啡因

发布时间：2018-02-28 02:58

本文关键词： 法医毒物分析 GC 甲基苯丙胺咖啡因生物样品　出处：《中国法医学杂志》2009年01期 　论文类型：期刊论文

【摘要】：目的建立同时测定血、尿中甲基苯丙胺和咖啡因含量的方法。方法应用GC/NPD技术,以4-苯基丁胺为内标,直接碱化,用氯仿提取,三氟乙酸酐衍生化,8CB熔融石英毛细管柱(30m×0.25mm×0.25μm)分析。结果生物样品中甲基苯丙胺与咖啡因在0.012~7.5μg/mL浓度范围内线性关系良好,检测限(S/N=3)依次为1.2ng/mL,0.6ng/mL(血);1.6ng/mL,0.8ng/mL(尿)。苯丙胺在0.017~10.0μg/mL浓度范围内线性关系良好,检测限为1.6ng/mL(血),3.2ng/mL(尿)。所有样本回收率均大于85%。结论本方法准确、灵敏,适用于血、尿中甲基苯丙胺及其代谢物苯丙胺的三氟乙酸酐衍生化物和咖啡因的同时检测,为判定滥用毒品种类、追查毒品来源以及研究生物体内甲基苯丙胺和咖啡因的交互影响提供了检测手段。
[Abstract]:Objective to establish a method for simultaneous determination of methamphetamine and caffeine in blood and urine. Methods by GC/NPD, 4-phenylbutylamine was directly alkalized and extracted by chloroform. Trifluoroacetic anhydride derivation was performed on a 30m 脳 0.25mm 脳 0.25 渭 m fused quartz capillary column. Results the linear relationship between methamphetamine and caffeine in biological samples was in the range of 0.012 渭 g / mL and 7.5 渭 g / mL, respectively. The detection limit was 1.2ng / mL0.6ng / mL. the linear range of phenylamphetamine was 0.017 渭 g / mL, and the detection limit was 1.6ng / mL (urine 3.2ng / mL). Conclusion the method is accurate, sensitive and applicable to blood, and the detection limit is 1.6 ng / mL. Conclusion the method is accurate, sensitive and applicable to blood, and the detection limit is 1.6 ng / mL. the recovery rate of all samples is more than 85ng / mL.Conclusion this method is accurate, sensitive and applicable to blood, and the detection limit is 1.6 ng / mL (blood 3.2ng / mL). Conclusion the method is accurate, sensitive and applicable to blood. Simultaneous determination of trifluoroacetic anhydride derivatives and caffeine in urine, methamphetamine and its metabolites, in order to determine the type of drug abuse, Tracing drug sources and studying the interactions of methamphetamine and caffeine in organisms provide a means of detection.
【作者单位】：四川大学华西药学院;四川大学华西基础医学与法医学院;
【分类号】：D919

【参考文献】