液-质联用法对人血浆中吗啡类、氯胺酮和苯丙胺类9种毒品的定性定量检测

发布时间：2018-03-13 23:04

本文选题：吗啡类　切入点：氯胺酮　出处：《中国运动医学杂志》2017年07期 　论文类型：期刊论文

【摘要】：目的:建立同时测定人血浆中吗啡、6-单乙酰吗啡、可待因、海洛因、氯胺酮、3,4-甲二氧基苯异丙胺、3,4-亚甲二氧基甲基安非他明、苯丙胺、甲基苯丙胺的定性定量检测方法。方法:血浆经10%三氯乙酸直接沉淀后进样分析。色谱柱为ACQUITY UPLC HSS C18柱,流动相为含0.01%甲酸的水溶液-0.01%甲酸的甲醇溶液,流速为0.3 m L·min-1,电喷雾离子源,用多反应监测,正离子分段扫描分析。结果:可用保留时间、特征碎片离子、分子离子峰丰度比为指标来定性鉴别血浆中9种化合物。另外,吗啡、可待因、海洛因、氯胺酮、6-单乙酰吗啡、3,4-甲二氧基苯异丙胺、3,4-亚甲二氧基甲基安非他明、苯丙胺、甲基苯丙胺血药浓度分别在5.00×10-3~5.00(r=0.9934),1.00×10-2~10.00(r=0.9905),1.00×10-2~10.00(r=0.9929),2.50×10-3~2.50(r=0.9960),5.00×10-3~5.00(r=0.9925),5.00×10-4~5.00(r=0.9910),5.00×10-4~5.00(r=0.9924),5.00×10-4~5.00(r=0.9920),5.00×10-4~5.00(r=0.9900)μg·m L-1内,线性关系均良好;最低检测限分别为1.00、1.00、1.00、0.50、0.50、0.10、0.10、0.10、0.10 ng·m L-1。日内、日间精密度(RSD)均18%;提取回收率均60%,RSD均15%。结论:该方法灵敏、快速、准确、专属性强,可用于吗啡类、氯胺酮和苯丙胺类9种毒品化合物的定性筛查和定量检测研究。
[Abstract]:Objective: to establish a simultaneous method for the determination of morphine 6-monoacetylmorphine, codeine, heroin, ketamine, 3-trimethoxyphenyl isopropylamine, 4-methylene dioxy methamphetamine and amphetamine in human plasma. Qualitative and quantitative determination of methamphetamine. Methods: plasma was precipitated with 10% trichloroacetic acid. The chromatographic column was ACQUITY UPLC HSS C18 column and the mobile phase was methanol solution containing 0.01% formic acid in aqueous solution -0.01% formic acid. The flow rate was 0.3 mL 路min-1, electrospray ion source, multi-reaction monitoring and positive ion segmental scanning analysis. Results: retention time, characteristic fragment ion and molecular ion peak abundance ratio can be used as indicators to qualitatively identify 9 compounds in plasma. The serum drug concentrations of morphine, codeine, heroin, ketamine, 6-monoacetylmorphine, methoxybenzylamine, methamphetamine, methamphetamine and methamphetamine were 5.00 脳 10-35.00, 0.99341.00 脳 10-210.0000r0.9905, 0.99292.50 脳 10-32.50p 0.9960605.00 脳 10-35.00rnr0.9925 脳 10-45.00r0 0.99105.00 0.99105.00 0.245.00 脳 10 ~ (-4) 脳 10 ~ (-4) 脳 10 ~ (-4) 脳 10 ~ (-400) 脳 0.995.00 渭 g 路L ~ (-1) and 0.9925 脳 10 ~ (-45.00) 渭 g 路min ~ (-1), 0.9925 脳 10 ~ (-4) 脳 10 ~ (-4) 脳 10 ~ (-5) 0 脳 10 ~ (-5) 0 脳 10 ~ (-5) 0 脳 10 ~ (-4) 脳 10 ~ (-4) 脳 10 ~ (-4) 脳 10 ~ (-4) 脳 10 ~ (-4) 脳 10 ~ (-5) O ~ (-1) and a good linear relationship (n = 0.9910 ~ (-1) ~ (-1)). The minimum detection limits were 1.00 ~ 1.00 ~ 1.00 ~ 1.00 ~ 0.50 ~ 0.50 ~ 0.50 ~ 0.10 ~ 0.10 ~ 0.10 ~ 0.10 ng 路m ~ (-1) 路m ~ (-1) 路min ~ (-1) 路d ~ (-1), and the recovery rate was 60% and RSD was 150.Conclusion: this method is sensitive, rapid, accurate and specific, and can be used for morphine. Qualitative screening and quantitative detection of nine kinds of ketamine and amphetamines.
【作者单位】：中国民用航空局民用航空医学中心(民航总医院);北京市公安局刑侦总队;
【基金】：中国民用航空局民航安全能力建设资金项目(DFS20160604)
【分类号】：D919.2

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