北京汉族群体30个常染色体InDel位点群体遗传学及法医学研究

发布时间：2018-03-21 02:08

本文选题：插入缺失多态性　切入点：Investigator汶DIPplex　出处：《遗传》2013年12期 　论文类型：期刊论文

【摘要】：为了调查30个InDel位点在中国北京汉族人群中的群体遗传学数据,并评估其法医学应用价值,文章采集210名北京汉族无关健康个体外周血样,提取样本DNA,采用Investigator汶DIPplex体系对HLD77等30个InDel位点进行复合扩增,ABI3130 XL遗传分析仪进行基因分型,计算常用法医学参数,分析群体遗传差异。经Bonferroni校正,30个InDel位点不存在连锁不平衡现象,基因型分布符合Hardy-Weinberg平衡;各位点DP值为0.2690~0.6330,累积个体识别力(TDP)为0.999999999985;三联体累积非父排除率(CPEtrio)为0.98771049,二联体累积非父排除率(CPEduo)为0.94579456。32例STR基因座发生突变的家系样本调查证实上述InDel位点未发现突变。结果表明,Investigator汶DIPplex试剂盒中包含的30个InDel位点在北京汉族群体中具有较好的遗传多态性,在STR存在突变及微量DNA检材等特殊检案中可作为有效的补充检测体系。
[Abstract]:In order to investigate the population genetic data of 30 InDel loci in Han population in Beijing, China, and to evaluate the value of forensic application, peripheral blood samples were collected from 210 unrelated healthy individuals of Han nationality in Beijing. The DNA samples were extracted, and 30 InDel sites such as HLD77 were amplified by Investigator Wen DIPplex system. The genotyping was performed by ABI3130XL genetic analyzer, and the common forensic parameters were calculated. After Bonferroni correction, there was no linkage disequilibrium at 30 InDel loci, and genotype distribution was consistent with Hardy-Weinberg equilibrium. The DP value of each point is 0.2690 / 0.6330, the cumulative individual recognition power is 0.999999999985. the cumulative non-paternal exclusion rate of triplet is 0.98771049, and the cumulative non-paternal exclusion rate is 0.94579456.32 STR loci mutation. The results showed that the 30 InDel loci contained in the DIPplex kit had good genetic polymorphisms in the Han population of Beijing. It can be used as an effective supplementary detection system in special cases such as mutation of STR and trace samples of DNA.
【作者单位】：中国政法大学证据科学教育部重点实验室;
【基金】：教育部新世纪优秀人才项目(编号:NCET-10-0773) 国家自然科学基金面上项目(编号:81172902) 中组部北京市优秀人才项目(编号:2012D002023000002) 中国政法大学青年教师学术创新团队项目资助
【分类号】：Q987;D919

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