人死亡后特定条件下骨髓细胞核DNA降解与死后时间间隔推断

发布时间：2018-04-15 03:17

本文选题：死后时间间隔 + DNA含量　；参考：《第四军医大学学报》2009年07期

【摘要】：目的:探讨人死亡后骨髓细胞核DNA含量变化与晚期死后时间间隔关系.方法:离体人胸骨置于特定条件下,随时间延长分别取材制成病理切片,应用DNA高度特异性染色方法Feulgen改良法结合计算机图像分析技术对死后不同时间胸骨骨髓细胞核DNA含量变化进行分析.结果:人死亡后1～10d,Feulgen染色阳性产物随死亡时间延长逐渐减少,胸骨骨髓细胞核DNA含量随死亡时间延长呈规律下降,DNA染色逐渐变浅淡,至死后10d已基本观察不到.结论:人死亡后骨髓细胞核DNA含量随死亡时间延长呈下降趋势,两者之间存在线性关系.
[Abstract]:Objective: to investigate the relationship between nuclear DNA content in bone marrow and postmortem interval after death.Methods: the isolated human sternum was placed under certain conditions, and the pathological sections were obtained with the extension of time.The changes of nuclear DNA content in sternum bone marrow at different time after death were analyzed by DNA highly specific staining method (Feulgen) and computer image analysis.Results: the positive products of Feulgen staining gradually decreased with the prolongation of death time, and the nuclear DNA content of sternal bone marrow decreased regularly with the prolongation of death time.Conclusion: the nuclear DNA content of bone marrow decreased with the prolongation of death time after death, and there was a linear relationship between the two.
【作者单位】：广东司法警官职业学院司法鉴定系;遵义医学院珠海校区;广东警官学院;
【基金】：广东省自然科学基金(036628)
【分类号】：D919.4

【相似文献】