自然条件下铜绿蝇蛹发育形态及其法医学意义

发布时间：2018-04-22 20:05

本文选题：铜绿蝇 + 法医昆虫学　；参考：《昆虫学报》2017年02期

【摘要】：【目的】研究自然条件下铜绿蝇Lucilia cuprina蛹期蛹壳颜色变化和蛹的形态发育规律从而为死后间隔时间(postmortem interval,PMI)的推断提供理论依据。【方法】在室外自然条件下饲养铜绿蝇直至其化蛹,每12 h取蛹10头,固定、观察,并利用图像分析系统收集并分析蛹壳颜色的变化信息;在体式显微镜下剥离蛹壳,观察蛹体外部形态变化并拍照记录,描述蛹期发育特征。【结果】自然条件下,铜绿蝇蛹壳颜色随时间呈现逐渐加深趋势,尤其在化蛹初期和临近羽化时变化明显,根据采集到的蛹壳颜色RGB值可做出标准色板。蛹的形态发育呈规律性变化,可将蛹期划分为8个阶段,包括隐头期、显头期、棕翅期、黑鬃胸期、黑鬃腹期、半红眼期、红眼期和预成虫期。【结论】在案发现场,蛹壳颜色标准色板可用于目视比色,初步粗略估算蛹期,同时依据蛹期发育阶段进程表可进一步推算化蛹后时间,从而推断PMI。
[Abstract]:[objective] to study the color changes of pupae shell and the morphological development of pupae during pupae stage of Lucilia cuprina under natural conditions so as to provide a theoretical basis for the inference of postmortem intervalval pupae (PMI). [methods] the pupae of Lucilia cuprina was raised under outdoor natural conditions until its pupation. 10 pupae were taken every 12 hours, fixed, observed, and the change information of pupa shell color was collected and analyzed by image analysis system, the pupae shell was stripped under a pose microscope, the changes of the pupae's external morphology were observed and photographed. [results] under natural conditions, the pupae shell color showed a trend of deepening with time, especially in the early stage of pupation and near emergence. The standard color plate could be made according to the collected RGB value of pupae shell color. The development of pupae is regularly changed, and the pupa stage can be divided into 8 stages, including hidden head stage, obvious head stage, brown wing stage, black bristle thoracic stage, black bristles ventral stage, semi-red eye stage, red eye stage and preadult stage. [conclusion] The standard color plate of pupa shell can be used for visual colorimetry, and the pupa period can be estimated roughly, and the pupae time after pupation can be further calculated according to the developmental stage schedule of pupa stage, thus the PMIs can be inferred.
【作者单位】：河北医科大学病原生物学教研室;河北医科大学第一医院骨科;河北医科大学临床学院教务科;
【分类号】：D919

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