噪声污染对大鼠生理生化水平的影响研究

发布时间：2018-05-09 21:59

本文选题：噪声污染 + 环磷酸腺苷　；参考：《甘肃政法学院》2017年硕士论文

【摘要】：噪声污染对人类及动物机体有着一定的影响,当下国内外研究主要侧重于噪声污染对听力系统的影响,或局限于某一系统,而对噪声污染的影响机制缺乏系统研究,因此本论文以Wistar大鼠为实验对象,取雄性Wistar大鼠40只,随机分为4组,每组10只。根据不同分贝的噪声,将大鼠分为38dB组、55 dB组、70 dB组和对照组,对照组正常饲养。实验组每天在固定时段给予相应噪声刺激,每天一次,每次30分钟,连续刺激21天。末次刺激后即刻用乙醚麻醉,无痛手术打开胸腔,从左心室进针,采血,用全自动生化分析仪检测大鼠血液成分含量的变化,用酶联免疫吸附测定法(Enzyme-linked immune sorbent assay,ELISA)检测大鼠血液中环磷酸腺苷(Cyclic adenosine phosphate,cAMP)、环磷酸鸟苷(Cyclic guanosine phosphate,cGMP)、热休克蛋白-70(Heat shock proteins,HSP-70)、睾酮(Testosterone,T)、多巴胺(Dopamine,DA)、去甲肾上腺素(Noradrenaline,NA)的含量的变化。本论文的主要研究结果如下:分别给予Wistar大鼠38dB、55 dB、70 dB刺激连续21天,血液生化检测结果发现,大鼠血液中球蛋白(Globulin)、血糖(Blood sugar)、甘油三酯(Triglycerides)、淀粉酶(Amylase)的水平随着噪声分贝的增加而升高。其中球蛋白水平比对照组分别升高了6.07%、18.21%、89.13%;血糖水平比对照组分别升高了502.67%、647.20%、874.93%;甘油三酯水平比对照组分别升高了5.54%、148.19%、347.95%;淀粉酶水平比对照组分别升高了18.59%、27.20%、87.69%。大鼠血液中的白蛋白(Albumin)、总胆红素(Total bilirubin)、肌酸激酶(Creatine kinase)、肌酐(Creatinine)、尿素(Urea)的含量随着噪声分贝的增加而降低。其中白蛋白水平比对照组分别降低了6.47%、9.07%、13.68%;总胆红素水平比对照组分别降低了28.89%、44.63%、71.85%;肌酸激酶水平比对照组分别降低了3.48%、20.37%、31.86%;肌酐水平比对照组分别降低了2.75%、16.10%、31.55%;尿素水平比对照组分别降低了17.51%、28.32%、45.83%。酶联免疫吸附测定法(enzyme-linked immune sorbent assay,ELISA)检测发现,实验组大鼠血液中热休克蛋白-70(HSP-70)水平比对照组分别升高了39.34%、195.09%、285.25%;环磷酸鸟苷(cGMP)水平比对照组分别升高了99.45%、115.71%、171.40%;环磷酸腺苷(cAMP)水平比对照组分别升高了27.64%、132.49%、270.79%;睾酮(T)水平比对照组分别升高了8.90%、22.87%、35.80%;去甲肾上腺素(NA)水平比对照组分别升高了34.12%、172.96%、198.49%;多巴胺(DA)水平比对照组分别升高了11.33%、24.00%、39.33%。通过以上实验结果可以得出,噪声污染能显著影响血液成分水平。血液中淀粉酶、血糖、甘油三酯、环磷酸腺苷、环磷酸鸟苷、热休克蛋白-70、睾酮、多巴胺、去甲肾上腺素水平随着噪声刺激强度的增加而升高;血液中肌酸激酶、尿素、肌酐水平降低。
[Abstract]:Noise pollution has a certain impact on human and animal organism. At present, the research at home and abroad mainly focuses on the impact of noise pollution on hearing system, or confined to a certain system, but there is no systematic research on the mechanism of noise pollution. Therefore, 40 male Wistar rats were randomly divided into 4 groups, 10 rats in each group. According to the noise of different decibels, the rats were divided into 38dB group (55 dB) and control group (70 dB). The experimental group was given corresponding noise stimulation once a day for 30 minutes every day for 21 days. Immediately after the last stimulation, the rats were anesthetized with ether, painless operation was performed to open the chest cavity, the needle was injected from the left ventricle, blood was collected, and the changes of blood components in rats were detected by automatic biochemical analyzer. The contents of cyclic adenosine monophosphate (adenosine) phosphatecAMPP, cyclic guanosine phosphatec GMPP, heat shock protein -70 heat shock protein HSP-70, testosterone Testosterone Thunb, dopamine DopamineDAN and noradrenaline NAA were determined by enzyme-linked immunosorbent assay (Elisa). The main results of this study are as follows: Wistar rats were stimulated with 38dBX 55 dB 70 dB for 21 days, and the results of blood biochemistry test showed that, The levels of globulin globulin, blood glucose, triglycerides triglycerides, amylase Amylase increased with the increase of noise decibels. Compared with the control group, the level of globulin increased 6.07% 18.21 and 89.13, the blood glucose level of the control group increased 502.67, 647.20 and 874.93, the triglyceride level increased 5.548.19 and 347.95, and the amylase level increased 18.597.2027.20 and 87.695.The level of triglyceride was higher than that of the control group, and the amylase level was higher than that of the control group, and the level of amylase was higher than the control group. The contents of albumin, total bilirubin, creatine kinase, creatinine, urea Urea in blood of rats decreased with the increase of noise decibels. Compared with the control group, the albumin level was decreased by 6.477.07 and 13.68; the total bilirubin level was reduced by 28.899.630.71.85, respectively; the creatine kinase level was reduced by 3.48m 20.370.370.31. 86; the creatinine level was decreased by 2.7516.101031.55and the urea level was lower than that of the control group by 17.51and 28.327.32% and 45.835.83respectively, compared with the control group, the serum albumin level was significantly lower than that of the control group (P < 0.05), and the total bilirubin level was lower than that of the control group (P < 0.01). Enzyme-linked immune sorbent assaysa assay revealed that, Compared with the control group, the levels of heat shock protein-70 (HSP-70) in the blood of the experimental group were 39.34 higher than those in the control group, respectively. The levels of cyclic guanosine monophosphate (cGMPP) increased 99.455,115.71R (171.40), the levels of adenosine monophosphate (cAMPs) increased 27.64242.494mg / 270.79, respectively, and the levels of testosterone were 270.79% higher than those of the control group. Compared with the control group, the level of norepinephrine was increased by 34.12 and 172.96, and the dopamine DAA was increased by 11.333.00 and 39.33, respectively, compared with the control group. From the above results, it can be concluded that noise pollution can significantly affect the level of blood components. The levels of amylase, blood glucose, triglyceride, cyclic adenosine monophosphate, cyclic guanosine monophosphate, heat shock protein 70, testosterone, dopamine, norepinephrine increased with the increase of noise intensity. Creatinine level decreased.
【学位授予单位】：甘肃政法学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：D919.4

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