急性乙二醇中毒的研究

发布时间：2018-06-27 04:58

本文选题：乙二醇 + 中毒　；参考：《重庆医科大学》2010年硕士论文

【摘要】： 目的:通过SD大鼠急性乙二醇中毒的研究,为法医病理学、法医毒物分析鉴定急性乙二醇中毒提供客观诊断指标。方法:本实验采用剂量为12ml/kg的乙二醇作为灌注剂量,对SD大鼠进行灌注,动物分为2组:正常对照组和急性中毒组。每组进行生化指标、毒化、组织病理及TEM观察。所有数据输入计算机并采用统计软件进行统计学分析。结果: 1.SD大鼠急性乙二醇中毒后,形态学上证实,心、肝、肾、脾等均有不同程度的损害,其中尤以肾损害最重。 2.各器官组织中均有明显血管损伤:血管内皮细胞肿胀脱落、细胞核拉长、深染,平滑肌变性、纤维素性坏死、伴炎细胞侵润,部分管腔内血栓形成。 3.在肝、肾组织学观察中,可见到淡蓝色结晶管型。在TEM观察,肾小管上皮胞浆内可见到呈圆形、椭圆形高密度电子颗粒,管腔内可见中等电子密度的物质沉积。 4.在脾脏内见到多核巨细胞,尤以中毒12天的SD大鼠中较多。 5.急性乙二醇中毒有明显肝、肾功能损害及电解质紊乱。 6.气相色谱法是一种快速、准确的乙二醇定性、定量方法,本实验检测的大鼠灌胃后2天内在相应保留时间有明显的乙二醇峰出现,且峰面积的大小与灌胃剂量成正相关,中毒4天、8天、12天组血清中未检出乙二醇。结论: 1.乙二醇中毒从生化指标及形态学证实存在心、肝、肾脏损伤、尤以肾脏损伤明显(靶器官)。 2.乙二醇中毒存在血管壁损伤。 3.乙二醇中毒在肾、肝(尤以肾脏明显)可见淡蓝色管型结晶。 4.乙二醇中毒脾脏内有多核巨细胞形成。 5.乙二醇中毒存在电解质紊乱。 6.乙二醇中毒毒物检测:中毒两天以内直接检测乙二醇,如存活时间超过2天以上则须检测其代谢缓慢的代谢产物乙醇酸。
[Abstract]:Objective: to provide an objective diagnostic index for forensic pathology and toxicological analysis of acute ethylene glycol poisoning in SD rats. Methods: SD rats were perfused with ethylene glycol (12ml/kg) as the perfusion dose. The rats were divided into two groups: normal control group and acute poisoning group. Biochemical indexes, toxicosis, histopathology and TEM were observed in each group. All data input computer and statistical software for statistical analysis. Results: 1. After acute ethylene glycol poisoning in SD rats, morphologically, the heart, liver, kidney, spleen and so on were found to have different degrees of damage, especially the kidney damage was the most serious. 2. There were obvious vascular injury in all organs and tissues: vascular endothelial cells swelling and shedding, nucleus elongating, deep staining, smooth muscle degeneration, cellulose necrosis, inflammatory cells invading and thrombosis forming in some lumen. Light blue crystalline tubules were observed in liver and kidney histology. Tem observation showed that in the cytoplasm of renal tubular epithelium, circular and elliptical high density electron particles could be seen, and the deposition of medium electron density could be seen in the lumen of renal tubular epithelium. 4. Multinucleated giant cells were found in the spleen, especially in SD rats which were poisoned for 12 days. Acute ethylene glycol poisoning has obvious liver, kidney function damage and electrolyte disturbance. 6. Gas chromatography is a rapid and accurate qualitative and quantitative method for ethylene glycol. The peak of ethylene glycol appeared in the corresponding retention time within 2 days after gastric perfusion in rats, and the peak area was positively correlated with the dose of gastric perfusion. No ethylene glycol was detected in serum of the group of 4 days and 8 days and 12 days of poisoning. Conclusion: 1. Ethylene glycol poisoning has been confirmed by biochemical indexes and morphology of heart, liver, kidney injury, especially in the kidney damage (target organ). 2. Ethylene glycol poisoning has vascular wall damage. 3. Ethylene glycol poisoning in the kidney, liver (especially the kidney) visible light blue tubular crystallization. 4. Multinucleated giant cells were formed in spleen after ethylene glycol poisoning. Electrolyte disturbance in ethylene glycol poisoning. 6. Ethylene glycol poisoning poison detection: poisoning within two days of direct detection of ethylene glycol, if the survival time of more than 2 days must be tested for its slow metabolite glycolic acid.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：D919.1

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