一步法RT-PCR检测人狂犬病毒初探

发布时间：2018-06-29 05:16

本文选题：法医物证学 + 人狂犬病　；参考：《中国法医学杂志》2008年06期

【摘要】：目的探讨一步法逆转录聚合酶链反应(one-step reverse transcriptase-PCR或一步法RT-PCR)在人狂犬病检测中的应用。方法4例临床诊断狂犬病死亡患者的冰冻脑组织,用一步法RT-PCR检测病毒,狂犬病毒CVS株感染的鼠脑和急性胰腺炎死者的脑分别作为阳性和阴性对照。结果4例脑组织中提取的RNA,电泳后得到与狂犬病N基因长度一致的片断。结论一步法RT-PCR可用于人狂犬病毒的检测,在法医学鉴定中具有重要意义。
[Abstract]:Objective to investigate the application of one step reverse transcription polymerase chain reaction (one-step reverse transcriptase-PCR or one step RT-PCR) in detection of human rabies. Methods the frozen brain tissues of 4 patients with clinically diagnosed rabies died were detected by one-step RT-PCR for the positive and negative control of the infected brain of rabies virus CVS strain and the dead patients with acute pancreatitis. Results the RNAs extracted from 4 brain tissues were sequenced to the same length as those of rabies N gene by electrophoretic electrophoresis. Conclusion One-step RT-PCR can be used for detection of human rabies virus, which is of great significance in forensic identification.
【作者单位】：华中科技大学同济医学院法医学系;中国科学院武汉病毒研究所病毒学国家重点实验室;中国生物制品集团公司武汉生物制品研究所基因工程室;
【基金】：国家自然科学基金资助项目(30570629)
【分类号】：D919

【相似文献】