死后细菌学检测在死因鉴定中的作用初探

发布时间：2018-07-29 14:47

【摘要】：目的:评价死后细菌学检测在死因鉴定中的作用。方法:取健康SD大鼠30只,10只采用颈椎脱臼法处死纳入A组,10只制作缺氧缺血模型20min确认死亡纳入B组,10只注射金黄色葡萄球菌纳入C组,不给于治疗,待死亡,分别在死后3、6、9日,取后肢深部肌肉,采用化学发光法检测ATP水平,另取30只SD大鼠,同法处死,分别在死后第1-10日,采样取后肢深部肌肉、肝脏、脾脏、肾脏组织,进行细菌学培养镜检。结果:以ATP浓度对数值作为横坐标,以相对发光单位的对数值作为横坐标,呈一元线性方程Y=0.815X+9.015,R2=0.998,P0.01;A组、B组、C组死后第3日、6日、9日ATP组间与组内比较差异具有统计学意义(P0.05);普通培养、厌氧培养,A组、B组、C组肌肉、肝脏、脾脏、肾脏细菌培养阳性出现时间、优势菌类型差存在差异。结论:死后细菌学检测可用于死因鉴定中,ATP、细菌培养与鉴定等都可作为死因判断依据,但检测结果受观察时间等因素影响,同时是否适用于野外大型动物死亡尚无明确定论。
[Abstract]:Objective: to evaluate the role of postmortem bacteriological examination in the identification of causes of death. Methods: 30 healthy Sprague-Dawley rats were killed by cervical dislocated method and 10 rats were killed in group A to make hypoxic-ischemic model 20min confirmed that 10 staphylococcus aureus were injected into group B to be included in group C, without treatment, pending death. The deep muscles of the hind limbs were taken from the deep muscles of the hind limbs and the levels of ATP were detected by chemiluminescence method. The other 30 SD rats were killed by the same method. The deep muscles, liver, spleen and kidney of the hind limbs were sampled on the 1-10 days after death. Microscopical examination of bacteriological culture was carried out. Results: the logarithm of ATP concentration was taken as the horizontal coordinate, and the logarithmic value of the relative luminous unit was taken as the transverse coordinate. There were significant differences between group C and group C on the 3rd, 6th and 9th day after death in group B (P 0.05), but in normal culture, anaerobic culture in group A, group C, muscle, liver, spleen in group C, while in group A, there was no significant difference between group B and group C (P 0.05), while in normal culture, anaerobic culture showed a significant difference between group A and group C (P < 0.05), while in group A, there were significant differences in muscle, liver and spleen between group A and group B (P 0.05). The time of positive culture of kidney bacteria was different, and the type of dominant bacteria was different. Conclusion: the postmortem bacteriological examination can be used in the identification of the cause of death, bacteria culture and identification can be used as the basis for the cause of death, but the results are affected by the observation time, and whether it is applicable to the death of large animals in the field is not clear.
【作者单位】：武宣县公安局;
【分类号】：D918.9

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