亚急性甲基苯丙胺中毒大鼠相关脑区Caspase-3和Bcl-2表达的研究
发布时间：2018-08-01 14:42
【摘要】： 目的:1.研究亚急性甲基苯丙胺中毒大鼠的一般行为学特点。2.研究Caspase-3和Bcl-2蛋白在甲基苯丙胺中毒损伤机制中所发挥的作用。 方法:40只健康雄性SD大鼠随机分为对照组(n=10)和实验组(又分为3个亚组,分别为末次注射后第1天、第4天、第7天组,n=10)。实验组给予20mg/kg的甲基苯丙胺腹腔注射,对照组给予相同剂量的生理盐水注射,每天注射两次,注射时间为8:00,20:00,连续4天。每次注射后观察动物的行为学改变。末次注射后分别于第1天、第4天、第7天分三批处杀。腹腔注射戊巴比妥钠麻醉大鼠后用4%多聚甲醛灌注,取脑后置于4%的多聚甲醛中固定,石蜡包埋,作冠状切片,行HE染色和免疫组化染色。用免疫组化技术对中毒大鼠相关脑区Caspase-3和Bcl-2的表达进行检测,用图像分析技术对检测结果进行分析。 结果:Caspase-3和Bcl-2在大鼠脑的不同区域表达变化规律基本相似,均可见较明显的阳性信号,Bcl-2在末次注射后第1天达到高峰,随后表达下降,Caspase-3在末次注射后第4天达到高峰,随后逐渐下降。Bcl-2峰值早于Caspase-3出现。 结论:1.亚急性甲基苯丙胺中毒动物模型成功建立。2.凋亡因子Caspase-3在甲基苯丙胺中毒大鼠相关脑区额叶皮质、纹状体、海马部位的异常表达,表明其参与了甲基苯丙胺神经毒性损伤。3.凋亡因子Bcl-2在甲基苯丙胺中毒大鼠相关脑区额叶皮质、纹状体、海马部位的异常表达,表明其与甲基苯丙胺神经毒性损伤保护机制密切相关。
[Abstract]:Purpose 1. To study the general behavioral characteristics of subacute methamphetamine poisoning rats. To study the role of Caspase-3 and Bcl-2 proteins in the mechanism of methamphetamine poisoning. Methods 40 healthy male Sprague-Dawley rats were randomly divided into control group (n = 10) and experimental group (n = 10). The experimental group was intraperitoneally injected with methamphetamine of 20mg/kg and the control group was injected with the same dose of normal saline twice a day for 8: 00 20: 00 for 4 days. Behavioral changes were observed after each injection. They were killed in three batches on day 1, day 4 and day 7 after the last injection. Rats anesthetized with pentobarbital sodium were injected intraperitoneally with 4% paraformaldehyde. The brain was fixed in 4% paraformaldehyde and embedded in paraffin. Coronal sections were made and HE staining and immunohistochemical staining were performed. The expression of Caspase-3 and Bcl-2 in the related brain regions of poisoned rats was detected by immunohistochemical technique and the results were analyzed by image analysis technique. Results the expression of Caspase-3 and Bcl-2 were similar in different regions of brain of rats. The positive signal of Bcl-2 reached its peak on the first day after the last injection, then the expression of Caspase-3 reached the peak on the 4th day after the last injection. The peak value of Bcl-2 was earlier than that of Caspase-3. Conclusion 1. The animal model of subacute methamphetamine poisoning was established successfully. The abnormal expression of apoptosis factor Caspase-3 in frontal cortex, striatum and hippocampus of rats with methamphetamine poisoning suggests that it is involved in the neurotoxicity injury of methamphetamine. The abnormal expression of apoptosis factor Bcl-2 in the frontal cortex, striatum and hippocampus of rats with methamphetamine poisoning suggests that it is closely related to the protective mechanism of methamphetamine neurotoxicity.
【学位授予单位】：昆明医学院
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：D919
本文编号：2157907
[Abstract]:Purpose 1. To study the general behavioral characteristics of subacute methamphetamine poisoning rats. To study the role of Caspase-3 and Bcl-2 proteins in the mechanism of methamphetamine poisoning. Methods 40 healthy male Sprague-Dawley rats were randomly divided into control group (n = 10) and experimental group (n = 10). The experimental group was intraperitoneally injected with methamphetamine of 20mg/kg and the control group was injected with the same dose of normal saline twice a day for 8: 00 20: 00 for 4 days. Behavioral changes were observed after each injection. They were killed in three batches on day 1, day 4 and day 7 after the last injection. Rats anesthetized with pentobarbital sodium were injected intraperitoneally with 4% paraformaldehyde. The brain was fixed in 4% paraformaldehyde and embedded in paraffin. Coronal sections were made and HE staining and immunohistochemical staining were performed. The expression of Caspase-3 and Bcl-2 in the related brain regions of poisoned rats was detected by immunohistochemical technique and the results were analyzed by image analysis technique. Results the expression of Caspase-3 and Bcl-2 were similar in different regions of brain of rats. The positive signal of Bcl-2 reached its peak on the first day after the last injection, then the expression of Caspase-3 reached the peak on the 4th day after the last injection. The peak value of Bcl-2 was earlier than that of Caspase-3. Conclusion 1. The animal model of subacute methamphetamine poisoning was established successfully. The abnormal expression of apoptosis factor Caspase-3 in frontal cortex, striatum and hippocampus of rats with methamphetamine poisoning suggests that it is involved in the neurotoxicity injury of methamphetamine. The abnormal expression of apoptosis factor Bcl-2 in the frontal cortex, striatum and hippocampus of rats with methamphetamine poisoning suggests that it is closely related to the protective mechanism of methamphetamine neurotoxicity.
【学位授予单位】：昆明医学院
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：D919
【参考文献】
相关期刊论文 前1条
1 ;Intermittent hypoxia attenuates ischemia/reperfusion induced apoptosis in cardiac myocytes via regulating Bcl-2/Bax expression[J];Cell Research;2003年05期
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