延边朝鲜族和汉族人群15个常染色体STR基因座遗传多态性

发布时间：2018-08-22 19:20

【摘要】：目的:通过对中国吉林省延边地区朝鲜族人群与汉族人群常染色体上CSF1PO、D7S820、D8S1179、D21S11、D2S1338、D3S1358、D13S317、D16S539、TH01、D18S51、D19S433、TPOX、vWA、D5S818、FGA 这十五个 STR基因座的检测和数据分析,评价其法医学价值,为法医学个体识别、亲权鉴定、人类遗传学及其他相关学科提供参考数据。方法:研究对象选取延边朝鲜族无血缘个体血样224例,延边汉族无血缘个体血样407例,全部血样均按照Chelex-100方法来进行DNA的提取,获取的DNA 样本通过 AmpFlSTR(?)Identifiler(?)Plus PCR Amplification Kit 扩增常染色体中十五个STR基因座。扩增后产物的检测使用ABI310型DNA序列分析仪来完成,检测出来的数据通过GeneScan Analysis3.7和Genetyper3.7这两种基因分析软件来处理。针对研究中涉及的十五个STR基因座的多态性参数按照Modified-Powerstates标准版的计算模式来处理。同时,选择中国境内6个民族群体以及5个外国民族群体常染色体中的这十五个STR基因座进行对比分析,为群体遗传学的研究提供基础数据资料。根据POPTREE2软件的Neighbour-Joining(N-J)法设计的研究模式,按照等位基因频率来确定遗传距离,最终形成完整的系统进化树。结果:延边朝鲜族人群中的15个STR基因座共检出等位基因157个,基因频率在0.001-0.513之间;共检出基因型415种,基因型频率在0.004-0.317之间;杂合度分布在0.625-0.866之间,平均杂合度为0.7732,多态信息含量(PIC)分布在0.57-0.85之间,个人识别力(DP)在0.800-0.966之间,累计个人识别力(TDP)大于0.999999999,非父排除率(EP)分布在0.322-0.727之间,累计非父排除率(CEP)大于0.99999729延边汉族人群中的15个STR基因座共检出等位基因158个,基因频率在0.001-0.546之间;共检出基因型531种,基因型频率在0.002-0.309之间;杂合度分布在0.604-0.853之间,平均杂合度为0.7712,多态信息含量(PIC)分布在0.55-0.85之间,个人识别力(DP)在0.790-0.966之间,累计个人识别力(TDP)大于0.999999999,非父排除率(EP)分布在0.296-0.700之间,累计非父排除率(CEP)大于0.99999763中国吉林省延边地区的朝鲜族与汉族这两个民族群体在进行遗传距离的分析中可以看出,周边其他民族群体与朝、汉两民族群体之间的遗传距离要明显小于地理位置更远的其他民族群体。延边朝鲜族和汉族与国内外11个民族群体的系统进化树结果显示延边朝鲜族与韩国之间的亲缘关系最近。结论:延边朝鲜族和汉族的15个STR基因座中除TH01、D3S1358与T0PX外其它均显示高度多态性遗传性,适合朝鲜族和汉族群体法医学鉴定。延边朝鲜族、汉族和其他群体之间均存在不同程度的差异性,可阐明建立群体数据库的必要性。
[Abstract]:Objective: to evaluate the forensic value of 15 STR loci of CSF1POP D7S820 D8S1179, D21S1138 D3S1358, D16S539, TH01S51D19S433TPOXVWAD5S818FGA, based on the detection and data analysis of 15 STR loci in Korean and Han populations in Yanbian area, Jilin Province, China, and to evaluate the forensic value of D21S11S1338, D13S517, D16S539 and TH01S51D19S433TPOXVWAXD5S818FGA. Human genetics and other related disciplines provide reference data. Methods: two hundred and twenty-four unrelated individuals of Yanbian Korean nationality and 407 Han nationality of Yanbian Han nationality were selected to extract DNA according to Chelex-100 method. Fifteen STR loci of autosomes were amplified by AmpFlSTR (?) Identifiler (?) Plus PCR Amplification Kit) from the obtained DNA samples. ABI310 DNA sequence analyzer was used to detect the amplified products. The detected data were processed by GeneScan Analysis3.7 and Genetyper3.7. The polymorphic parameters of 15 STR loci involved in the study were treated according to the calculation model of Modified-Powerstates Standard Edition. At the same time, the 15 STR loci of 6 ethnic groups and 5 foreign ethnic groups in China were selected for comparative analysis, which provided basic data for the study of population genetics. According to the research model designed by Neighbour-Joining (N-J) method of POPTREE2 software, genetic distance is determined according to allele frequency, and a complete phylogenetic tree is formed. Results: a total of 157 alleles were detected in 15 STR loci in Yanbian Korean population, with a frequency of 0.001-0.513, a total of 415 genotypes (0.004-0.317), heterozygosity between 0.625 and 0.866, a total of 415 genotypes (0.004-0.317) and heterozygosity (0.625-0.866). The average heterozygosity was 0.7732, the polymorphic information content (PIC) was between 0.57-0.85, the personal recognition power (DP) was between 0.800-0.966, the cumulative personal recognition power (TDP) was greater than 0.99999999 9, and the non-paternal exclusion rate (EP) was 0.322-0.727. The cumulative non-paternal exclusion rate (CEP) of 15 STR loci in Yanbian Han population was greater than 0.99999729, with a total of 158 alleles with a gene frequency of 0.001-0.546, a total of 531 genotypes with genotype frequencies ranging from 0.002-0.309, and heterozygosity between 0.604-0.853. The average heterozygosity was 0.7712, the polymorphic information content (PIC) was between 0.55-0.85, the personal recognition power (DP) was between 0.790-0.966, the cumulative personal recognition power (TDP) was greater than 0.99999999 9, and the non-paternal exclusion rate (EP) was 0.296-0.700. The cumulative non-paternal exclusion rate (CEP) of the Korean and Han ethnic groups in Yanbian region of Jilin Province, China, is greater than 0.99999763. In the analysis of genetic distance, it can be seen that the surrounding ethnic groups and the DPRK, The genetic distance between Han and Han ethnic groups is obviously smaller than that of other ethnic groups with farther geographical location. The phylogenetic tree of Yanbian Korean and Han ethnic groups and 11 ethnic groups at home and abroad showed that Yanbian Korean nationality had the closest phylogenetic relationship with Korea. Conclusion: the 15 STR loci of Yanbian Korean and Han nationality showed highly polymorphic inheritance except TH01D3S1358 and T0PX, which are suitable for forensic identification of Korean and Han populations. There are some differences among Korean, Han and other groups in Yanbian, which can clarify the necessity of establishing group database.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：D919

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