牛四核苷酸STR基因座的筛选和多态性分析
发布时间：2018-10-14 14:47
【摘要】：目的筛选阴影带出现率低且多态性较高的牛四核苷酸STR基因座。方法用Tandem repeats finder软件搜索牛基因组中的四核苷酸重复STR序列片段,用Primer Premier 5.0软件设计引物,然后扩增、电泳,筛选出符合要求的STR基因座,并对100份无关黄牛个体血样进行STR基因座分型。结果共筛选出6个具有多态性的牛四核苷酸重复STR基因座(B006、B007、B008、B022、B025、B026),其100份无关黄牛个体血样的累积个体识别率和累积非父排除概率分别为0.99995和0.859591。结论本研究筛选出的6个四核苷酸STR基因座阴影带出现率低且多态性较高,可用于牛的个体识别和亲子鉴定的研究。
[Abstract]:Objective to screen bovine tetranucleotide STR loci with low frequency of shadow bands and high polymorphism. Methods Tandem repeats finder software was used to search repeated STR sequences in bovine genome, primers were designed with Primer Premier 5.0 software, then amplified, electrophoresis was used to screen the STR loci which met the requirements. STR locus typing was performed on 100 unrelated bovine blood samples. Results A total of 6 polymorphic STR loci of bovine tetranucleotide repeats (B006B007, B008, B022, B025, B026) were screened. The cumulative individual identification rates and cumulative non-paternal exclusion rates of 100 unrelated bovine blood samples were 0.99995 and 0.8595991, respectively. Conclusion the six tetranucleotide STR loci screened in this study have low occurrence rate and high polymorphism, which can be used in individual identification and paternity identification of cattle.
【作者单位】： 中山大学中山医学院法医物证学教研室;
【基金】：国家自然科学基金资助项目(30400519) 教育部留学回国人员科研启动基金资助项目(2008-890)
【分类号】：D919
[Abstract]:Objective to screen bovine tetranucleotide STR loci with low frequency of shadow bands and high polymorphism. Methods Tandem repeats finder software was used to search repeated STR sequences in bovine genome, primers were designed with Primer Premier 5.0 software, then amplified, electrophoresis was used to screen the STR loci which met the requirements. STR locus typing was performed on 100 unrelated bovine blood samples. Results A total of 6 polymorphic STR loci of bovine tetranucleotide repeats (B006B007, B008, B022, B025, B026) were screened. The cumulative individual identification rates and cumulative non-paternal exclusion rates of 100 unrelated bovine blood samples were 0.99995 and 0.8595991, respectively. Conclusion the six tetranucleotide STR loci screened in this study have low occurrence rate and high polymorphism, which can be used in individual identification and paternity identification of cattle.
【作者单位】： 中山大学中山医学院法医物证学教研室;
【基金】：国家自然科学基金资助项目(30400519) 教育部留学回国人员科研启动基金资助项目(2008-890)
【分类号】：D919
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