全血中氯霉素的固相萃取方法
发布时间：2018-10-25 13:32
【摘要】：目的建立全血中氯霉素的固相萃取方法。方法在空白血中添加标准氯毒素,采用HLB、MCX固相柱萃取,HPLC法测定了线性范围、精密度、回收率。结果回收率分别为:HLB为69.1%,RSD为6.21%,MCX为70.1%,RSD为4.34%。标准工作曲线Y=18.1094X-0.4822,相关系数r=0.9999,线性范围1.0~20.0ug/L,最小检出量3ng。标准添加工作曲线Y=-2.1165X+14.0459,相关系数r=0.9996,最小检出限0.5μg/mL(S/N=10∶1)。结论此二种萃取柱均可用于氯霉素的固相萃取。
[Abstract]:Objective to establish a solid phase extraction method for chloramphenicol in whole blood. Methods the standard chlorine toxin was added to the blank blood. The linear range, precision and recovery were determined by HLB,MCX solid phase column extraction. Results the recoveries were 69.1%, 6.21%, 70.1% and 4.34%, respectively. The standard working curve was 18.1094 X-0.4822, the correlation coefficient was 0.9999, the linear range was 1.0 ~ 20.0ugr / L, and the minimum detectable amount was 3ng. The standard added working curve was Y ~ (-2.1165) X 14.0459, the correlation coefficient was 0.9996, and the minimum detection limit was 0.5 渭 g/mL (S/N=10:1). Conclusion these two extraction columns can be used for solid phase extraction of chloramphenicol.
【作者单位】： 北京市公安局刑侦总队法医处;山西医科大学法医学院;
【分类号】：D919.1
[Abstract]:Objective to establish a solid phase extraction method for chloramphenicol in whole blood. Methods the standard chlorine toxin was added to the blank blood. The linear range, precision and recovery were determined by HLB,MCX solid phase column extraction. Results the recoveries were 69.1%, 6.21%, 70.1% and 4.34%, respectively. The standard working curve was 18.1094 X-0.4822, the correlation coefficient was 0.9999, the linear range was 1.0 ~ 20.0ugr / L, and the minimum detectable amount was 3ng. The standard added working curve was Y ~ (-2.1165) X 14.0459, the correlation coefficient was 0.9996, and the minimum detection limit was 0.5 渭 g/mL (S/N=10:1). Conclusion these two extraction columns can be used for solid phase extraction of chloramphenicol.
【作者单位】： 北京市公安局刑侦总队法医处;山西医科大学法医学院;
【分类号】：D919.1
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