X-STR和常染色体STR在二联体亲权鉴定中的应用
发布时间：2018-12-14 08:58
【摘要】：目的探讨X-STR和常染色体STR在二联体亲权鉴定中的应用价值。方法分别采用Sinofiler试剂盒和Mentype Argus X-8试剂盒对104例二联体亲权鉴定的样本DNA进行基因分型,并对各个STR基因座在排除案例中的应用价值进行统计分析。结果Sinofiler试剂盒和Mentype汶Argus X-8试剂盒对其中102例案件均可获得相同的鉴定结论;另外2例案件中,分别在DXS10074和DXS10035基因座检见突变可能。在69例排除案例中,Sinofiler检测系统中以D8S1179基因座的排除率最高,为56.52%;Mentype汶Argus X-8检测系统中以DXS10135基因座的排除率最高,达到85.51%。结论X-STR在法医学中具有很好的应用价值,不仅可以成为常染色体STR的重要补充,而且可以解决一些特殊案例的鉴定难题。
[Abstract]:Objective to investigate the application value of X-STR and autosomal STR in diad paternity determination. Methods Sinofiler kit and Mentype Argus X-8 kit were used to analyze the genotyping of 104 DNA samples of diad paternity test, and the application value of each STR locus in exclusion cases was analyzed statistically. Results both the Sinofiler kit and the Mentype Argus X-8 kit obtained the same identification results in 102 cases, while in the other two cases, mutations were detected in DXS10074 and DXS10035 loci, respectively. In 69 exclusion cases, the exclusion rate of D8S1179 locus was the highest in Sinofiler detection system, and the highest exclusion rate was DXS10135 locus in 56.52% Mentype Wen Argus X-8 detection system, which reached 85.51%. Conclusion X-STR has a good application value in forensic medicine. It can not only be an important supplement to autosomal STR, but also solve the problem of identification of some special cases.
【作者单位】： 苏州大学医学部法医学系;司法部司法鉴定科学技术研究所;
【基金】：上海市科技攻关课题项目(08251203200) 上海市自然科学基金(08ZR1419800)
【分类号】：D919.4
本文编号：2378338
[Abstract]:Objective to investigate the application value of X-STR and autosomal STR in diad paternity determination. Methods Sinofiler kit and Mentype Argus X-8 kit were used to analyze the genotyping of 104 DNA samples of diad paternity test, and the application value of each STR locus in exclusion cases was analyzed statistically. Results both the Sinofiler kit and the Mentype Argus X-8 kit obtained the same identification results in 102 cases, while in the other two cases, mutations were detected in DXS10074 and DXS10035 loci, respectively. In 69 exclusion cases, the exclusion rate of D8S1179 locus was the highest in Sinofiler detection system, and the highest exclusion rate was DXS10135 locus in 56.52% Mentype Wen Argus X-8 detection system, which reached 85.51%. Conclusion X-STR has a good application value in forensic medicine. It can not only be an important supplement to autosomal STR, but also solve the problem of identification of some special cases.
【作者单位】： 苏州大学医学部法医学系;司法部司法鉴定科学技术研究所;
【基金】：上海市科技攻关课题项目(08251203200) 上海市自然科学基金(08ZR1419800)
【分类号】：D919.4
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1 董春楠;四个X-STR基因座四色荧光复合分型体系构建及群体遗传学调查[D];河北医科大学;2011年
2 陈鹏宇;山西汉族X-STR基因座DXS9902、DXS7132遗传多态性及法医学应用[D];山西医科大学;2008年
3 冯文艳;山西汉族人群DXS7423、DXS6799基因座遗传多态性及法医学应用可行性探讨[D];山西医科大学;2009年
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