乌头碱对新生大鼠心肌细胞DNA损伤的影响
发布时间：2018-12-15 01:23
【摘要】：目的探讨乌头碱对新生大鼠心肌细胞DNA损伤的影响。方法新生SD大鼠20只,随机分为4组,取心室肌以差速贴壁法原代培养心室肌细胞。培养第6天,制成密度为2×105个细胞/mL的细胞悬液,分别加入乌头碱混合液至终浓度为0.1、0.5、1、2μmol/L,染毒30m in;采用彗星电泳技术及CASP分析软件,检测不同浓度乌头碱染毒后心室肌细胞DNA损伤程度。结果心肌细胞被不同浓度乌头碱染毒后,尾部DNA含量、彗尾长度、尾矩、O live尾矩均随乌头碱浓度增加而升高,头部DNA含量则逐渐降低,与对照组相比,有显著性差异(P0.01);乌头碱染毒剂量越大,心肌细胞DNA损伤越严重。结论乌头碱染毒引起细胞DNA断裂损伤呈明显的剂量—效应关系,推测细胞DNA损伤是乌头碱毒作用机制之一。
[Abstract]:Objective to investigate the effect of aconitine on DNA damage in neonatal rat cardiomyocytes. Methods Twenty newborn SD rats were randomly divided into 4 groups. Ventricular myocytes were cultured with differential adhesion method. On the 6th day of culture, cell suspensions with density of 2 脳 105 cells / mL were prepared, and aconitine mixture was added to the final concentration of 0. 1 0. 5 渭 mol/L, for 30 m in;. Comet electrophoresis and CASP software were used to detect the degree of DNA damage in ventricular myocytes exposed to aconitine at different concentrations. Results the contents of DNA, tail length and tail moment of aconitine increased with the increase of aconitine concentration, and the content of DNA in the head decreased gradually, compared with the control group. There was significant difference (P0.01). The greater the dose of aconitine was, the more serious the DNA damage of cardiomyocytes was. Conclusion there is a dose-effect relationship between aconitine and aconitine in the DNA damage induced by aconitine. It is speculated that DNA damage is one of the mechanisms of aconitine toxicity.
【作者单位】： 华中科技大学同济医学院法医学系;湖北省中山医院病理科;
【基金】：湖北省自然科学基金资助项目(2008CDB206)
【分类号】：D919
[Abstract]:Objective to investigate the effect of aconitine on DNA damage in neonatal rat cardiomyocytes. Methods Twenty newborn SD rats were randomly divided into 4 groups. Ventricular myocytes were cultured with differential adhesion method. On the 6th day of culture, cell suspensions with density of 2 脳 105 cells / mL were prepared, and aconitine mixture was added to the final concentration of 0. 1 0. 5 渭 mol/L, for 30 m in;. Comet electrophoresis and CASP software were used to detect the degree of DNA damage in ventricular myocytes exposed to aconitine at different concentrations. Results the contents of DNA, tail length and tail moment of aconitine increased with the increase of aconitine concentration, and the content of DNA in the head decreased gradually, compared with the control group. There was significant difference (P0.01). The greater the dose of aconitine was, the more serious the DNA damage of cardiomyocytes was. Conclusion there is a dose-effect relationship between aconitine and aconitine in the DNA damage induced by aconitine. It is speculated that DNA damage is one of the mechanisms of aconitine toxicity.
【作者单位】： 华中科技大学同济医学院法医学系;湖北省中山医院病理科;
【基金】：湖北省自然科学基金资助项目(2008CDB206)
【分类号】：D919
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