单体素~1H-MRS推断死亡时间的初步研究

发布时间：2018-12-23 12:09

【摘要】：目的检测脑内N-乙酰天门冬氨酸、胆碱复合物及磷酸肌酸和肌酸的变化规律,并探讨其与死亡时间的关系。方法选取12只白兔,利用单体素氢质子磁共振波谱测定死后0.5、1、2、4、6、8、12、16、24h脑内N-乙酰天门冬氨酸、胆碱复合物及磷酸肌酸和肌酸峰下面积;分析其在上述时间点的变化规律,建立与死亡时间相关的回归方程。结果在死后24h内N-乙酰天门冬氨酸、磷酸肌酸和肌酸峰下面积随死亡时间的延长而降低(各时间点比较P0.05),胆碱复合物在死后24h内无明显变化(各时间点比较P0.05)。回归方程为:Naa:y=0.7425x2-27.282x+299.984(R2=0.962);Cr:y=0.3234x2-12.007x+210.503(R2=0.986)。结论死后24h内兔脑N-乙酰天门冬氨酸、磷酸肌酸和肌酸呈现出一定的变化规律,其与死亡时间相关程度较高,可用于对一定条件下死亡时间的推断。
[Abstract]:Objective to detect the changes of N-acetylaspartate, choline complex, creatine phosphate and creatine in the brain, and to explore the relationship between N- acetyl aspartate, choline complex, creatine phosphate and time of death. Methods A total of 12 white rabbits were selected to measure the area under the peak of N-acetylaspartate, choline complex, creatine phosphate and creatine. The law of change at the above time point is analyzed, and the regression equation related to the time of death is established. Results the area of N- acetyl aspartate, creatine phosphate and creatine peak decreased with the prolongation of death time within 24 hours after death (P0.05). There was no significant change in choline complex within 24 hours after death (P0.05 compared with each time point). The regression equation was: Naa:y=0.7425x2-27.282x 299.984 (R _ 2N _ (0.962) and Cr:y=0.3234x2-12.007x _ (210.503) (R _ (2) O _ (0. 986). Conclusion N- acetyl aspartic acid, creatine phosphate and creatine in the brain of rabbits showed a certain change within 24 hours after death, and the correlation between N- acetyl aspartate, creatine phosphate and creatine was relatively high, which could be used to infer the time of death under certain conditions.
【作者单位】：华中科技大学同济医学院法医学系;华中科技大学同济医学院附属同济医院放射科;
【分类号】：D919

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