顶空固相微萃取-气相色谱-质谱联用法测定尿液中美沙酮及其主要代谢物
发布时间：2019-04-23 23:43
【摘要】：提出了用顶空固相微萃取-气相色谱-质谱联用法测定尿液中美沙酮及其主要代谢物2-亚乙基-1,5-二甲基-3,3-二苯基吡咯烷(EDDP)的含量。对影响萃取效率的萃取温度、萃取时间、pH以及离子强度等因素进行了试验,并予以优化。在优化的条件下,采用二苯基丙基己酸β-二己基氨基乙酯(SKF_(525A))为内标,选定m/z 277,m/z 72和m/z 86分别作为EDDP、美沙酮和SKF_(525A)的定量离子,在选择离子监测模式下,美沙酮及EDDP的线性范围均为0.01～1.0 mg·L~(-1),相对标准偏差(n=5)都小于9.0%,平均回收率分别为104.2%和97.6%。
[Abstract]:A headspace solid-phase microextraction-gas chromatography-mass spectrometry method was developed for the determination of methadone and its main metabolite 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) in urine by headspace solid-phase microextraction (SPME)-gas chromatography-mass spectrometry (GC-MS). The factors affecting extraction efficiency, such as extraction temperature, extraction time, pH and ionic strength, were tested and optimized. Under the optimized conditions, 尾-dihexylaminoethyl diphenylpropylcaproate (SKF_ (525A) was used as the internal standard, and the EDDP, methadone and SKF_ (525A) were selected as EDDP, methadone and SKF_ (525A), respectively. The linear range of methadone and EDDP was 0.01 ~ 1.0 mg 路L ~ (- 1), the relative standard deviation (n = 5) was less than 9.0%, the average recoveries of methadone and methadone were 104.2% and 97.6%, respectively.
【作者单位】： 广西公安厅物证鉴定中心;广西大学化学化工学院;广西公安厅科技处;
【基金】：公安部应用创新计划项目资助项目(2007YYCXGXST081)
【分类号】：D919.2
本文编号：2463913
[Abstract]:A headspace solid-phase microextraction-gas chromatography-mass spectrometry method was developed for the determination of methadone and its main metabolite 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) in urine by headspace solid-phase microextraction (SPME)-gas chromatography-mass spectrometry (GC-MS). The factors affecting extraction efficiency, such as extraction temperature, extraction time, pH and ionic strength, were tested and optimized. Under the optimized conditions, 尾-dihexylaminoethyl diphenylpropylcaproate (SKF_ (525A) was used as the internal standard, and the EDDP, methadone and SKF_ (525A) were selected as EDDP, methadone and SKF_ (525A), respectively. The linear range of methadone and EDDP was 0.01 ~ 1.0 mg 路L ~ (- 1), the relative standard deviation (n = 5) was less than 9.0%, the average recoveries of methadone and methadone were 104.2% and 97.6%, respectively.
【作者单位】： 广西公安厅物证鉴定中心;广西大学化学化工学院;广西公安厅科技处;
【基金】：公安部应用创新计划项目资助项目(2007YYCXGXST081)
【分类号】：D919.2
【共引文献】
相关期刊论文 前1条
1 郑克勤;李洪;刘淮;;对海洛因成瘾者社区药物维持治疗试点门诊今后发展方向的思考[J];皮肤病与性病;2006年04期
相关硕士学位论文 前1条
1 刘庆艳;三相液相微萃取—高效液相色谱测定体液中滥用物质研究[D];湖南师范大学;2007年
【相似文献】
相关硕士学位论文 前1条
1 潘智文;生物检材中的毒品及其代谢物的气相色谱—质谱联用检测[D];广西大学;2008年
，本文编号：2463913
本文链接：https://www.wllwen.com/falvlunwen/fanzuizhian/2463913.html
