母婴分离所致的慢性内脏痛对成年大鼠空间学习记忆能力和旷场行为的影响

发布时间：2018-01-02 14:04

本文关键词：母婴分离所致的慢性内脏痛对成年大鼠空间学习记忆能力和旷场行为的影响　出处：《神经解剖学杂志》2016年03期 　论文类型：期刊论文

【摘要】：目的:观察母婴分离所致的慢性内脏痛对成年大鼠空间学习记忆能力和旷场行为的影响。方法:模型组大鼠于出生后第3~21 d,每天接受3 h母婴分离,对照组大鼠不做分离处理;8周龄时,使用腹壁撤退反射评分和痛阈测定方法筛选出有慢性内脏痛的大鼠;Morris水迷宫检测成年大鼠的空间学习记忆能力;旷场实验评估成年大鼠的旷场行为;Western Blot方法检测大鼠海马脑源性神经营养因子(brain derived neurotrophic factor,BDNF)的表达变化。结果:(1)母婴分离成年大鼠分别在20、40、60、80 mm Hg压力下的腹壁撤退反射评分均显著高于对照大鼠(P0.05),而其痛阈显著低于对照大鼠(P0.05)。(2)与对照组相比,母婴分离大鼠水迷宫的寻台潜伏期与平均平台象限滞留时间均无显著差异(P0.05)。(3)与对照大鼠相比,母婴分离大鼠旷场运动的总路程和总速度无显著差异(P0.05),但中心路程和中心路程时间显著减少(P0.05)。(4)Western Blot结果显示母婴分离大鼠海马内BDNF的表达与对照相比显著降低(P0.05)。结论:母婴分离所致的慢性内脏痛对成年大鼠空间学习记忆能力和自主探究行为无显著影响,但能引发成年大鼠的心理焦虑,该效应可能与海马BDNF的表达减少有关。
[Abstract]:Objective: to observe the effects of chronic visceral pain induced by maternal and infant separation on spatial learning and memory ability and open field behavior in adult rats. The rats in the control group received 3 hours of maternal and infant separation every day, and the rats in the control group were not treated with separation. At the age of 8 weeks, rats with chronic visceral pain were selected by abdominal wall withdrawal reflex score and pain threshold test. The spatial learning and memory ability of adult rats was measured by Morris water maze. Open field experiment was used to evaluate the open field behavior of adult rats. Western Blot method was used to detect brain derived neurotrophic factor in hippocampus of rats. Results the expression of BDNF in adult rats separated from mother and infant was 20 ~ 40 ~ 60 respectively. The abdominal wall withdrawal reflex score under 80 mm Hg pressure was significantly higher than that of the control rats (P 0.05), and the pain threshold was significantly lower than that of the control rats (P 0.05). There was no significant difference between the delay-seeking latency and the average platform quadrant retention time in the water maze of mother-infant separation rats. There was no significant difference in the total distance and the total velocity of the open-field movement between the mother and the child separated rats (P0.05). The results of Western Blot showed that the expression of BDNF in hippocampus of rats with maternal and infant separation was significantly lower than that of control group (P 0.05). Conclusion: chronic visceral pain caused by maternal and infant separation has no significant effect on spatial learning and memory ability and autonomous inquiry behavior of adult rats. But it can induce psychological anxiety in adult rats, which may be related to the decrease of BDNF expression in hippocampus.
【作者单位】：福建医科大学基础医学院生理学与病理生理学系老化与神经变性疾病重点实验室;福建医科大学基础医学院;
【基金】：国家自然科学基金(81471138) 福建省自然科学基金(2013J01127,2014J01124) 福建省教育厅基金(JA12149)
【分类号】：R402
【正文快照】： 慢性疼痛不仅是一种简单的病理生理现象,而且也是伴随着情绪、认知和行为改变的一种疾病[1]。近年来,越来越多的临床研究表明,慢性疼痛病人的不良情绪如焦虑、抑郁和恐惧等所带来的危害远大于疼痛本身[2]。肠易激综合征(irritable bowel syn-drome,IBS)是临床常见的肠道功能紊

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