泰州市腹泻病人中致泻大肠埃希氏菌感染状况及病原学特征分析

发布时间：2018-01-04 17:17

本文关键词：泰州市腹泻病人中致泻大肠埃希氏菌感染状况及病原学特征分析　出处：《现代预防医学》2016年02期 　论文类型：期刊论文

【摘要】：目的了解泰州市腹泻病人中致泻大肠埃希氏菌感染状况、毒力基因、分子分型以及耐药性情况,为致泻大肠埃希氏菌防治提供依据。方法对2013年泰州市215份腹泻病人粪便标本进行多重PCR鉴定和分离培养,并对分离的致泻大肠埃希氏菌菌株进行生化鉴定、脉冲场凝胶电泳(Pulsed Field Gel Electrophoresis,PFGE)分子分型以及药敏试验。结果 215份腹泻病人粪便标本共检测出致泻大肠埃希氏菌30株,其中EPEC 20株(占9.30%),EAEC 5株(占2.33%),ETEC 5株(占2.33%);PFGE结果表明,30株致泻大肠埃希氏菌PFGE带型种类多,各亲缘关系较近,但无100%的同源菌,同时PFGE分析结果表明在同一个粪便中有2种不同致泻大肠埃希氏菌感染情况存在;药敏试验表明,30株致泻大肠埃希氏菌对阿莫西林、四环素耐药性最高,3耐以及以上占63.33%。结论泰州市腹泻病人中致泻大肠埃希氏菌感染普遍存在,并以EPEC、EAEC、ETEC感染为主,菌株的耐药性较强,耐药谱广;基因型呈多态分布,且具有流行相关性。
[Abstract]:Objective to understand the infection status of diarrhea caused by Escherichia coli, patients with diarrhea in Taizhou in virulence gene, molecular typing and drug resistance, to provide the basis for prevention and treatment of diarrhea induced by Escherichia coli. Methods in Taizhou city in 2013 215 stool specimens were identified by multiplex PCR and cultured, and Enterovirulent Escherichia coli strains isolated the biochemical identification, pulsed field gel electrophoresis (Pulsed Field Gel Electrophoresis, PFGE) molecular typing and drug sensitivity test. Results 215 stool specimens were detected in 30 strains of Escherichia coli. Among them, 20 strains of EPEC (9.30%), 5 strains of EAEC (2.33%), ETEC (5 strains 2.33%); PFGE results showed that 30 strains of Escherichia coli. PFGE with many kinds of the close genetic relationship, but no 100% strains, and PFGE analysis results show that in the same stool in 2 different diarrheogenic Escherichia coli Presence of Hilbert infection; drug sensitivity test showed that 30 strains of Escherichia coli. To amoxicillin, tetracycline resistance and resistance to the highest, accounting for more than 3 common diarrhea caused by Escherichia coli infection 63.33%. Taizhou conclusion diarrhea patients, and in EPEC, EAEC, ETEC infection, strains with strong resistance, resistance broad spectrum; genotypes were polymorphic distribution, and has a popular correlation.

【作者单位】：泰州市疾病预防控制中心;中国疾病预防控制中心传染病预防控制所;
【基金】：泰州市软科学研究项目(RM201420)
【分类号】：R446.5
【正文快照】： 致泻大肠埃希氏菌是一类能够引起人和动物肠炎和腹泻的大肠杆菌[1]。在发展中国家,致泻大肠埃希氏菌是引起儿童腹泻病的主要原因,也是主要的公共卫生健康问题原因之一[1,2]。根据致泻大肠埃希氏菌携带毒力基因不同将其分为5类[1,3]:肠产毒性大肠杆菌(ETEC)、肠致病性大肠杆菌(

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