三种速冻方法制备新鲜冰冻血浆的比较分析

发布时间：2018-01-05 12:07

本文关键词：三种速冻方法制备新鲜冰冻血浆的比较分析　出处：《中国输血杂志》2016年01期 　论文类型：期刊论文

【摘要】：目的分析不同速冻方法制备的新鲜冰冻血浆制剂的质量差异,探讨适合血站使用的制备血浆的速冻方法。方法分别采用血浆速冻机速冻法、速冻冰箱平摆速冻法、速冻冰箱竖直速冻法3种方法速冻新鲜冰冻血浆,并随机抽取每种方法的20袋血浆进行血浆蛋白含量(TP)、血浆纤维蛋白原含量(Fg)、凝血因子Ⅷ(FⅧ)含量、凝血因子Ⅴ(FⅤ)含量检测。结果 3种方法速冻过程的中心温度降低到-30℃的平均时间(min)分别为23.7±0.7、216.8±2.2、432.9±3.1,差异有统计学意义(P0.05);血浆速冻机速冻法、速冻冰箱平摆速冻法、速冻冰箱竖直速冻法制备的新鲜冰冻血浆FⅤ含量(IU/m L)分别为1.13±0.1、0.98±0.13、0.84±0.09,FⅧ含量(IU/m L)分别为1.15±0.16、0.93±0.11、0.79±0.10,差异均有统计学意义(P0.05),TP含量(g/L)分别为64.17±5.32、64.06±4.67、63.92±5.23,差异无统计学意义(P0.05);3种方法制备的Fg含量(g/L)分别为2.52±0.22、2.31±0.23、2.37±0.26,速冻机速冻法与速冻冰箱,平摆速冻放置和竖直放置这2种方法制备的Fg含量差异有统计学意义(P0.05),后2种方法制备的Fg含量(g/L)差异无统计学意义(P0.05)。结论冷冻速率和血浆中心温度是制备新鲜冰冻血浆的2个关键参数,应建立科学的血浆速冻方法,保障血浆产品的疗效。
[Abstract]:Objective to analyze the different preparation methods of frozen fresh frozen plasma preparation to investigate the differences in the quality of the frozen plasma preparation method for blood use. Methods the plasma freezer frozen, frozen refrigerator swing quick freezing method, frozen frozen refrigerator vertical frozen by 3 kinds of methods of fresh frozen plasma, and were randomly selected from each method 20 bags of plasma plasma protein content (TP), plasma fibrinogen (Fg), coagulation factor VIII (F) content, coagulation factor V (F V) were detected. The results of 3 methods of freezing the center temperature drop to the average time of -30 C (min) were 23.7 + 0.7216.8 + 2.2432.9 + 3.1, the difference was statistically significant (P0.05); plasma freezer frozen, frozen refrigerator swing frozen, frozen refrigerator frozen vertical prepared fresh frozen plasma F V (IU/m L) content were 1.13 + 0.1,0.98 + 0.13,0.84 + 0.09, F (IU/m L) content were 1.15 + 0.16,0.93 + 0.11,0.79 + 0.10, the differences were statistically significant (P0.05), the content of TP (g/L) were 64.17 + 5.32,64.06 + 4.67,63.92 + 5.23, the difference was not statistically significant (P0.05); Fg was prepared by 3 methods (g/L) were 2.52 + 0.22,2.31 + 0.23,2.37 + 0.26, freezer frozen and frozen refrigerator, there was a significant difference in Fg content of swing vertically placed and frozen this prepared by 2 methods (P0.05), the content of Fg after prepared by 2 methods (g/L) showed no significant difference (P0.05). Conclusion the freezing rate and plasma temperature are the 2 key parameters for preparation of fresh frozen plasma, frozen plasma should establish a scientific method, guarantee the efficacy of plasma products.

【作者单位】：北京市通州区中心血站;张家口市中心血站;
【基金】：北京市科技工作基础专项基金(2013004)
【分类号】：R457.14
【正文快照】： 新鲜冰冻血浆(fresh frozen plasma,FFP)的疗效和质量与全血采集后储存时间、温度和血浆制备流程等因素有关。目前采供血机构一般采用血浆速冻机和速冻冰箱这2种设备制备新鲜冰冻血浆。《血站技术操作规程》(2012年版)规定:血浆制备时最好在60 min内将血浆中心温度降至-30℃以

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