Bruker MS与VITEK-MS鉴定近平滑念珠菌复合体的性能对比

发布时间：2018-01-06 13:00

本文关键词：Bruker MS与VITEK-MS鉴定近平滑念珠菌复合体的性能对比　出处：《中华医院感染学杂志》2016年10期 　论文类型：期刊论文

【摘要】：目的评估中国医院侵袭性真菌监测网(CHIF-NET)10和11收集的近平滑念珠菌复合体原始鉴定、Bruker MS与VITEK MS鉴定结果。方法经中心实验室分子生物学方法复核鉴定确认,CHIF-NET 10和11从全国22所医院共收集到近平滑念珠菌复合体352株,包括近平滑念珠菌305株,似平滑念珠菌36株,拟平滑念珠菌11株;以复核鉴定结果为金标准,评价各单位原始常规方法鉴定结果,并评估Bruker MS和VITEK MS基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)对近平滑念珠菌复合体的鉴定性能。结果与分子生物学鉴定结果相比,近平滑念珠菌的各医院原始鉴定准确率为90.8%,9.2%的菌株被API 20C或VITEK-2错误鉴定为光滑念珠菌、季也蒙念珠菌等;常规方法无法鉴定似平滑念珠菌和拟平滑念珠菌;Bruker MS和VITEK MS对305株近平滑念珠菌鉴定准确率分别达到了98.4%和97.7%;Bruker Biotyper 3.0数据库中有似平滑念珠菌和拟平滑念珠菌参考图谱,但鉴定准确率仅分别为33.3%和27.3%;VITEK MS V2.0没有两种菌的参考图谱而无法鉴定。结论该项研究率先在国内评估了Bruker MS和VITEK MS鉴定近平滑念珠菌复合体的性能,证明二者对于近平滑念珠菌的鉴定是比较可靠的。
[Abstract]:Objective to evaluate the Chinese hospital invasive fungal monitoring network (CHIF-NET) original c.parapsilosis complex identification of 10 and 11 were collected, Bruker MS and VITEK MS. The identification results confirmation method by the Central Laboratory of molecular biology methods review identification, CHIF-NET 10 and 11 from 22 hospitals across the country were collected 352 strains of Candida parapsilosis complex, including nearly 305 strains of Candida parapsilosis, like Candida paropsilosis in 36 strains, 11 strains of Candida to smooth; to review identification results as the gold standard, the evaluation results of conventional methods of each unit of original identification, and evaluation of Bruker MS and VITEK MS matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) on the performance appraisal of c.parapsilosis complex results. Compared with the results of molecular identification, the accuracy rate of each hospital c.parapsilosis original identification for 90.8%, 9.2% strains were identified as API 20C or VITEK-2 smooth read error Zhuhai bacteria, Candida guilliermondii; conventional methods can not be identified as Candida parapsilosis and Candida Bruker MS and quasi smooth; VITEK MS on the accuracy of identification of 305 strains of Candida parapsilosis respectively 98.4% and 97.7%; Bruker Biotyper 3 database like Candida parapsilosis and Candida parapsilosis Ni Ping reference map, but the identification accuracy rate respectively. 33.3% and 27.3%; VITEK MS V2.0 no reference map of two kinds of bacteria which could not be identified. Conclusion this study evaluated the performance of the first in the domestic Bruker MS and VITEK MS identification of Candida parapsilosis complex, proving the two identification for Candida parapsilosis is reliable.

【作者单位】：中国医学科学院北京协和医院检验科;中国医学科学院研究生院;
【基金】：国家自然科学基金资助项目(81572057)
【分类号】：R446.5
【正文快照】： 近年来随着生命支持系统的广泛应用,近平滑念珠菌复合体已成为念珠菌血症第二常见的病原菌[1]。近平滑念珠菌复合体可引起多种临床相关感染,显著增加住院患者的病死率和医疗支出。2005年以前,近平滑念珠菌分为3种不同基因型(Ⅰ、Ⅱ和Ⅲ型)[2]。实验室使用常规方法是不能够将近

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