血细胞分离机不同采集程序对外周血造血干细胞分离效果的影响

发布时间：2018-01-07 15:18

本文关键词：血细胞分离机不同采集程序对外周血造血干细胞分离效果的影响　出处：《中国实验血液学杂志》2016年05期 　论文类型：期刊论文

【摘要】：目的:观察COBE Spectra血细胞分离机Auto PBSC程序、MNC程序及Spectra Optia血细胞分离机MNC程序采集自体外周血造血干细胞的效果及不良反应。方法:对41例采集对象分别采用COBE Spectra血细胞分离机Auto PBSC程序、MNC程序及Spectra Optia血细胞分离机MNC程序进行自体外周血造血干细胞采集,观察3种采集程序采集的MNC及CD34~+细胞数、采集后患者血红蛋白及血小板下降的差异以及3种采集程序采集过程中的不良反应。结果:在全血处理量及采集时间基本相同的情况下,COBE Spectra和Spectra Optia的MNC程序采集的MNC数较COBE Spectra Auto PBSC程序采集的数高,但采集的CD34~+细胞数均低于Auto PBSC程序采集的CD34~+细胞数(P0.05)。COBE Spectra和Spectra Optia的MNC程序采集的终产物体积大于Auto PBSC程序采集的终产物体积。Spectra Optia血细胞分离机MNC程序与COBE Spectra血细胞分离机MNC程序相比,采集的M NC数无显著差异,但Spectra Optia血细胞分离机M NC程序采集的CD34~+细胞数大于COBE Spectra血细胞分离机MNC程序采集的CD34~+细胞数(P0.05)。Spectra Optia的MNC程序采集后患者血小板及血红蛋白较采集前下降幅度最低(P0.05)。3种程序采集过程中的不良反应相似,患者均可耐受。结论:COBE Spectra血细胞分离机Auto PBSC程序和Spectra Optia血细胞分离机MNC程序采集自体外周血造血干细胞优于COBE Spectra血细胞分离机的MNC程序。Spectra Optia的MNC程序采集后患者血小板及血红蛋白损失最低。
[Abstract]:Objective: To observe the COBE Spectra blood cell separator Auto PBSC program, MNC program and Spectra Optia blood cell separator MNC procedures for collection of autologous peripheral blood stem cells and adverse reaction effect. Methods: the COBE Spectra blood cell separator Auto PBSC procedure in 41 cases of acquisition object, MNC program and Spectra Optia blood cell separator MNC application of autologous peripheral blood stem cell collection, the number of MNC and CD34~+ cells 3 acquisition program acquisition of the observed differences in the adverse reactions after the collection of hemoglobin and platelet in patients with decreased and 3 kinds of acquisition program acquisition process. Results: in the same amount of blood processing and acquisition time, the number of MNC MNC data acquisition program COBE Spectra and Spectra Optia COBE Spectra Auto PBSC is the number of data acquisition program is high, but the number of CD34~+ cells were lower than the Auto PBSC acquisition program acquisition CD34~+ fine Cell number (P0.05) and Spectra Spectra end product volume.COBE Optia MNC program Auto PBSC acquisition program acquisition is greater than the end product volume.Spectra Optia blood cell separator MNC program and COBE Spectra blood cell separator MNC program compared with no significant difference between the number of M NC acquisition, but the number of CD34~+ cells Spectra Optia M NC blood cell separator the program collected more than COBE Spectra blood cell separator MNC acquisition program, CD34~+ cell count (P0.05).Spectra Optia MNC program after the collection of platelets and hemoglobin were collected before the lowest decline (P0.05) adverse reaction of.3 program in the process of collecting similar, can be tolerated. Conclusion: COBE Spectra blood cell separator Auto PBSC program Spectra and Optia blood cell separator MNC acquisition program, autologous peripheral blood stem cells than COBE Spectra blood cell separator MNC program.Spectra Opt The loss of blood platelets and hemoglobin in patients with ia's MNC program was the lowest.

【作者单位】：西安交通大学第一附属医院血液内科;
【基金】：陕西省自然科学基础研究计划青年基金(2016JQ8057)
【分类号】：R457.7
【正文快照】： Auto PBSC程序procedure of COBE Spectra and M NC procedure of Spectra Optia are better than M NC procedure of COBE Spectra forautologous peripheral blood hematopoietic stem cells collection.The loss of blood platelet and hemoglobin after collec-tion is lo

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