解脲脲原体生物群分布与耐药性差异研究

发布时间：2018-01-07 15:23

本文关键词：解脲脲原体生物群分布与耐药性差异研究　出处：《中国人兽共患病学报》2016年01期 　论文类型：期刊论文

更多相关文章： 解脲脲原体 生物群 血清型 耐药性

【摘要】：目的了解本地区泌尿生殖道感染人群中解脲脲原体感染的生物群分布特点及其耐药情况。方法采用液体选择培养基对泌尿道分泌物标本进行培养、鉴定及药敏;选取解脲脲原体阳性标本进行PCR分群和分型;分析各生物群型别及其耐药性差异。结果 985例检测标本中,单独解脲脲原体阳性395例,总阳性率为40.10%,其中女性368例,男性27例,其阳性率分别为42.45%、22.88%,女性阳性率高于男性,其差异有显著统计学意义(χ2=16.550,P0.01)。395例单独解脲脲原体阳性标本作PCR分群分型显示,单独生物一群阳性278例,阳性率为70.89%;单独生物二群阳性80例,阳性率为20.25%。单独生物一群中以单一血清6型(121例,43.21%)、3/14型(96例,35.53%)感染为主。单独生物二群均为混合血清型感染,基因亚型1(血清型2、5、8、9)和亚型3(血清型7、11)混合感染(49例,61.25%);基因亚型2(血清型4、10、12、13)和亚型3(血清型7、11)混合感染共检出(31例,38.75%)。结合药敏结果发现,解脲脲原体对喹诺酮类药物耐药率较高,对四环素类药物较敏感,且多重耐药现象严重。生物一群对司帕沙星的耐药性高于生物二群,二者差异有统计学意义(χ2=5.701,P=0.0170.05),且生物一群对米诺环素、多西环素、阿奇霉素的耐药性远高于生物二群,其差异有显著统计学意义(χ2=10.811,χ2=19.103,χ2=7.541,P均0.01)。进一步结合分型结果发现,生物一群中单一血清型S6耐药率最高的抗菌药为氧氟沙星(76.86%),S1型耐药率最高的抗菌药为罗红霉素(95.74%),S3/14型耐药率最高的抗菌药为罗红霉素和阿奇霉素(均为86.46%)。结论泌尿生殖道感染人群中解脲脲原体阳性率较高,女性高于男性,以生物一群单一血清型感染为主;本地区耐药形势严峻,生物一群耐药性高于生物二群;生物一群中各血清型的耐药性存在不同程度的差异。结合解脲脲原体生物分群分型结果可辅助合理选用抗菌药物,以避免滥用致耐药株产生。
[Abstract]:Objective to study the urogenital tract infection among Ureaplasma urealyticum infection distribution and drug resistance of biota. Methods using liquid selective medium for urinary tract secretions specimens for culture, identification and drug sensitivity; selection of Ureaplasma urealyticum positive specimens were divided PCR group and type; analyzes the different types and drug resistance of biota results. The 985 specimens, separate Ureaplasma urealyticum positive 395 cases, the total positive rate was 40.10%, there were 368 female and 27 male patients, the positive rates were 42.45%, 22.88%, the positive rate of female is higher than male, the difference was statistically significant (2=16.550, P0.01).395 single cases of Ureaplasma urealyticum the positive samples were PCR serotyping alone showed a group of creatures were positive in 278 cases, the positive rate was 70.89%; two separate biological group was positive in 80 cases, the positive rate was 20.25%. alone a group of creatures in a single serotype 6 (121 cases , 43.21%), 3/14 (96 cases, 35.53%) infection. Separate biovar two are mixed serotype infection, genotype 1 (serotype 2,5,8,9) and subtype 3 (serotype 7,11) mixed infection (49 cases, 61.25%); gene subtype 2 (serotype 4,10,12,13) and sub type 3 (serotype 7,11) mixed infection were detected (31 cases, 38.75%). According to the results of drug sensitivity showed that Ureaplasma urealyticum of quinolone resistance rate is higher, more sensitive to tetracycline, and multidrug resistant organisms. The serious phenomenon of a group of sparfloxacin was higher than that of biovar two and two with significant difference (2=5.701, P=0.0170.05), and a group of creatures of minocycline, doxycycline, azithromycin resistance is much higher than the two biological group, and the difference was statistically significant (x 2=10.811, 2=19.103 x, X 2=7.541, P 0.01). A combination of classification results showed that single serotype biological group S6 The highest resistance rate of antibacterial drugs for ofloxacin (76.86%), S1 the highest resistance rate of antibacterial drugs for Roxithromycin (95.74%), S3/14 the highest resistance rate of antibacterial drugs for roxithromycin and azithromycin (86.46%). The high positive rate of Ureaplasma urealyticum among genitourinary tract infection conclusion, female is higher than male, infection in a group of biological single serotype; local drug resistance situation is grim, a group of biological resistance than biological two groups; there are differences between different serotypes of drug resistance in a group of creatures. The combination of Ureaplasma urealyticum biological groups genotyping results can assist the rational use of antimicrobial drugs to avoid abuse caused by the production of antibiotic resistant strains.

【作者单位】：解放军252医院;
【分类号】：R446.5
【正文快照】： 解脲脲原体是一种介于病毒与细菌之间、能够独立存在的微生物。近年来其感染发生率呈逐年上升趋势,成为引起泌尿生殖道感染的重要病原体。目前已知解脲脲原体包括两个生物群[1],14个血清型:生物一群即微小解脲脲原体(Ureaplasm par-vum,Up),生物二群即解脲解脲脲原体(Ureaplas

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