利奈唑胺体外诱导粪肠球菌V区突变及耐药相关性研究

发布时间：2018-01-17 16:33

本文关键词：利奈唑胺体外诱导粪肠球菌V区突变及耐药相关性研究　出处：《第三军医大学学报》2016年08期 　论文类型：期刊论文

【摘要】：目的观察利奈唑胺是否能在体外诱导肠球菌产生高水平耐药,同时探索其耐药机制与23S rRNA的V区突变的关系,以及突变的基因拷贝数是否与耐药程度(MIC值)相关。方法 5株耐药机制未明的利奈唑胺低水平耐药粪肠球菌(MIC:4~16μg/m L)持续给予利奈唑胺进行体外诱导,观察其耐药水平变化;采用PCR技术扩增诱导菌和ATCC 29212的23S rRNA的V区片段以及编码核糖体蛋白L3、L4基因片段,分析有无V区和L3、L4突变及V区突变拷贝数。结果通过利奈唑胺持续诱导,5株耐药菌的MIC值较原菌株获得8~32倍增加,最高MIC值超过256 mg/L;其中有3株菌23S rRNA的V583区发生G2576T突变,其余2株菌未发现V区突变;所有诱导菌未发现L3、L4核糖体蛋白氨基酸改变。结论利奈唑胺在体外可诱导粪肠球菌MIC值迅速增加,其V区G2576T突变与高水平耐药密切相关,且突变拷贝数量与MIC值增高呈正相关。
[Abstract]:Objective to investigate whether lenazolidomide can induce high level drug resistance of Enterococcus in vitro and to explore the relationship between the drug resistance mechanism and V region mutation of 23s rRNA. And whether the copy number of mutant gene was related to the drug resistance degree and MIC value. Methods five strains of low level resistance-resistant Enterococcus faecalis, whose drug resistance mechanism is unknown, were studied. Linezolidomide was given continuously for in vitro induction. The level of drug resistance was observed. The V region fragment of 23s rRNA of inducible bacteria and ATCC 29212 and the gene fragment encoding ribosomal protein L3G L4 were amplified by PCR, and the V region and L3 gene were analyzed. L4 mutation and V region mutation copy number. Results the MIC value of 5 drug-resistant strains induced by linazolamide was increased by 832 times as compared with the original strain, and the highest MIC value was more than 256mg / L; G2576T mutation was found in the V583 region of 23s rRNA in 3 strains, and no mutation in V region was found in the other 2 strains. No changes of ribosomal protein amino acids were found in all induced bacteria. Conclusion the MIC value of Enterococcus faecalis can be increased rapidly by linazolamide in vitro. The V region G2576T mutation was closely related to the high level of drug resistance, and the number of mutant copies was positively correlated with the increase of MIC value.
【作者单位】：重庆医科大学附属第一医院检验科;
【基金】：国家自然科学基金面上项目(81572055)~~
【分类号】：R446.5
【正文快照】： 利奈唑胺作为第1个恶唑烷酮类抗生素,能与细菌核糖体23S rRNA结合,阻止翻译起始物的形成,对革兰阳性球菌引起的感染,包括耐甲氧西林金黄色葡萄球菌(methicillin resistant Staphylococcus aureus,MRSA)和耐万古霉素肠球菌(vancomycine resistantenterococci,VRE)具有良好的抗

【相似文献】