双抗体夹心ELISA法检测石头鱼毒素

发布时间：2018-01-18 06:00

本文关键词：双抗体夹心ELISA法检测石头鱼毒素　出处：《军事医学》2016年06期 　论文类型：期刊论文

【摘要】：目的建立石头鱼毒素neoVTX的酶联免疫吸附试验(ELISA)检测方法,为该毒素的检测、中毒诊断及治疗提供依据。方法以过碘酸钠法偶联马抗-HRP,利用双抗体夹心ELISA方法检测neoVTX。结果该法的线性范围在磷酸盐缓冲液(PBS)中为4~512 ng/ml,线性回归方程为Y=1.676X-1.140(r~2=0.9728,n=8,P0.0001),在血浆中检测线性范围为4~2048 ng/ml,线性回归方程为Y=0.3094X-0.1208(r~2=0.9907,n=10,P0.0001)。牛血清白蛋白、卵清蛋白及人血浆对检测结果无干扰。结论成功建立了石头鱼毒素的双抗体夹心ELISA检测方法,该法有较高的灵敏度和特异性,适用于溶液中及血浆中微量neoVTX的样品分析。
[Abstract]:Objective to establish an enzyme linked immunosorbent assay (Elisa) for the detection of stonefish toxin neoVTX. Methods the method of sodium periodate combined with horse anti-HRP was used to provide evidence for the diagnosis and treatment of poisoning. A double antibody sandwich ELISA method was used to detect neoVTX.Results the linear range of this method was 4 512 ng/ml in phosphate buffer solution. The linear regression equation was YT 1.676X-1.140C 0.9728NM (P0.0001A), and the linear range of detection in plasma was 4m 2048 ng/ml. The linear regression equation was YT 0.3094 X-0.1208 ru 20.9907 ~ (10) P0.00010.Bovine Serum Albumin (BSA). Ovalbumin and human plasma do not interfere with the detection results. Conclusion A double antibody sandwich ELISA method for the detection of stonefish toxin has been successfully established. The method has high sensitivity and specificity. It is suitable for sample analysis of trace neoVTX in solution and plasma.
【作者单位】：军事医学科学院生物工程研究所;
【基金】：国家863计划资助项目(SS2013AA090204)
【分类号】：R446.61
【正文快照】： 石头鱼(Synanceia)属辐鳍类亚纲(Actinoptery-gii)湁形目(Scorpaeniformes)毒湁科(Synanceidae),主要存在于太平洋和印度洋的热带水域,被认为是毒性最强的鱼类之一[1]。其背部的毒刺引起的刺伤,可引起剧痛、低血压、水肿、恶心、头晕、胸闷、肌无力以及呼吸和循环衰竭,甚至死

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