不同提取方法对粪便中短链脂肪酸含量及稳定性的影响

发布时间：2018-01-25 23:46

本文关键词： 短链脂肪酸稳定性高效液相色谱法操作分类单元　出处：《现代预防医学》2016年11期 　论文类型：期刊论文

【摘要】：目的测定粪便中乙酸、丙酸、丁酸和戊酸等短链脂肪酸的含量,研究不同提取方法和不同计算方法对SCFA含量和稳定性的影响。方法采用HPLC法测定粪便中SCFA的含量,采用高通量测序方法测定粪便菌群。结果在超纯水提取法中,以粪便湿重计算SCFA含量时,第4天测定的丙酸含量与第1天相比具有较大差异(t=-4.30,P=0.05);以OUT计算时,第4天测定的丁酸含量与第2天相比具有显著差异(t=5.63,P=0.03),而以属数目计算时,第4天测定的丙酸含量显著高于第1天(t=7.03,P=0.02)。在硫酸过滤法中,当以OUT计算时,第2天测定的丁酸含量高于第1天(t=-4.67,P=0.04),第4天测得的总SCFA含量低于第2天(t=5.98,P=0.03);而分别以湿重和属数目计算的各SCFA含量间均无显著差异。对超纯水提取法和硫酸过滤法进行比较后发现,两种提取方法间无显著差异。结论两种SCFA提取方法间无统计学差异,但采用硫酸过滤法提取、以粪便湿重计算时获得的SCFA含量具有较好的稳定性。
[Abstract]:Determination of acetic acid to propionic acid, feces, short chain fatty acid content of butyric acid and valeric acid. The effects of different extraction methods and different calculation methods on the content of SCFA and stability. A method for determination of fecal SCFA by HPLC method, using high-throughput sequencing method for determination of fecal bacteria. Results in ultrapure water extraction method. The stool wet weight of SCFA was calculated, and the propionic acid content was measured fourth days first days compared with large differences (t=-4.30, P=0.05); in the OUT calculations, fourth days of the determination of acid content and second day compared with significant difference (t=5.63, P=0.03), and to calculate the number of genera, the propionic acid content was measured fourth days the first day was significantly higher than that of (t=7.03, P=0.02). In sulfuric acid filtration method, when using OUT calculation, butyric acid content was measured second days more than first days (t=-4.67, P=0.04), the total SCFA content of fourth days measured less than second days (t=5.98, P=0.03); and respectively There were no significant differences between the wet weight and the calculation of the number of SCFA content. The ultrapure water extraction method and sulfuric acid filtration method were compared, no significant difference between the two kinds of extraction methods. Conclusion two kinds of SCFA extraction method had no significant difference, but the filtration method in sulfuric acid extraction, fecal SCFA content get wet when the weight calculation has good stability.

【作者单位】：深圳市疾病预防控制中心;中国科学院深圳先进技术研究院食品安全与环境技术研究室;
【基金】：国家外国专家局外传千人计划项目(项目编号:外专办发[2014]49号) 中国科学院-外国专家特聘研究员计划项目(项目编号:2013T1G0038) 深圳市科技计划项目(项目编号:GJHS20140901004635683)
【分类号】：R446.13
【正文快照】： 在人体肠道内,难于被直接消化的植物纤维、淀粉和低聚糖等可以被肠道细菌分解、发酵生产短链脂肪酸(Short Chain Fatty Acid,SCFA)[1-2],SCFA通常是指碳原子数目少于6个的有机酸,包括甲酸、乙酸、丙酸、丁酸、戊酸和异戊酸等。SCFA在人体肠道内具有重要作用,例如,丙酸能够影响

【参考文献】