2008-2014年北京市山夫登堡沙门菌耐药性及分子流行病学特征分析

发布时间：2018-02-08 14:06

本文关键词： 山夫登堡沙门菌耐药性脉冲场凝胶电泳分子分型　出处：《现代预防医学》2016年14期 　论文类型：期刊论文

【摘要】：目的分析北京市2008-2014年山夫登堡沙门菌临床分离株的分子流行病学特征。方法对从腹泻病例分离到的91株山夫登堡沙门菌进行药敏试验、脉冲场凝胶电泳(PFGE)分子分型及多位点序列分型(MLST)。结果 2011-2012年山夫登堡沙门菌在北京地区高水平流行,占所有分离株的87.9%,2008-2010年,2013-2104年各年份高度散发,病例主要集中在20~59岁成人组,5-6月为发病高峰;所有菌株对萘啶酸有较高耐药性(76.9%),对磺胺异VA唑(53.9%)和链霉素(49.5%)中度耐药,对其他抗菌药物均较敏感;PFGE分型将91株腹泻株分成23种型别,形成三大克隆群,引起2011-2012年暴发流行的64株菌形成绝对优势克隆株;42株菌的MLST分型结果显示包括暴发流行在内的绝大多数菌株属于常见型ST14,5株散发菌株为新型ST1923。结论北京市山夫登堡沙门菌具有明显的克隆株优势带型,引起2011-2012年的本地流行,对抗生素敏感性较高。
[Abstract]:Objective to analyze the molecular epidemiologic characteristics of clinical isolates of Salmonella Sanfendensi from 2008 to 2014 in Beijing. Methods Drug sensitivity tests were performed on 91 strains of Salmonella Sanfendensi isolated from diarrhea cases. Results the molecular typing and multilocus sequence typing of Salmonella Sanfendensi were highly prevalent in Beijing from 2011 to 2012, accounting for 87.9% of all isolates and highly distributed in 2013-2104 from 2008 to 2010. The cases were mainly concentrated in adults aged 20 to 59 years old in May to June, and all the strains had high resistance to naphthalic acid (76. 9%) and moderate resistance to sulfamethoxazole (53. 9%) and streptomycin (49. 5). 91 strains of diarrhea were classified into 23 types by PFGE typing for other antimicrobial agents, forming three clone groups. The MLST typing results of 64 strains that caused the outbreak in 2011-2012 showed that most of the strains, including the outbreak, belong to the common type ST14235.Conclusion the new type of ST1923was found in Shanfenden, Beijing. Salmonella albicans had obvious dominant band type of clone strain. Causes the 2011-2012 local epidemic, the antibiotic sensitivity is high.
【作者单位】：北京市疾病预防控制中心食物中毒诊断溯源技术北京市重点实验室;
【基金】：北京市高层次卫生技术人才培养计划(2013-3-099) “十三五”传染病国家科技重大专项(2016ZX10004215)
【分类号】：R446.5

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