56例不规则抗体引起ABO血型正反定型不相符的分析

发布时间：2018-02-12 10:34

  本文关键词： ABO血型鉴定 正反定型不相符 不规则抗体 特异性鉴定 临床输血　出处：《细胞与分子免疫学杂志》2016年02期 　论文类型：期刊论文

【摘要】：目的探讨引起ABO血型正反定型不相符的不规则抗体特异性及其免疫球蛋白类型。方法采用微柱凝胶法对患者进行ABO和Rh D血型鉴定,对ABO血型正反定型不相符的血标本再采用传统试管法进行复检,并采用微柱凝胶法进行不规则抗体筛选、特异性鉴定、抗体效价及免疫球蛋白类型检测。结果在引起ABO血型正反定型不相符的56例不规则抗体中,MNS血型系统19例(33.9%)、P血型系统1例(1.8%)、Lewis血型系统15例(26.8%)、Kidd血型系统4例(7.1%)、Rh血型系统10例(17.9%)、冷自身抗体7例(12.5%)。免疫球蛋白类型为Ig M型42例(75.0%),抗体效价为1∶16~1∶8192;Ig G型10例(17.9%),抗体效价为1∶128~1∶1024;Ig M、Ig G混合型4例(7.1%),抗体效价为1∶32~1∶256。37℃反应性:±~4~+。结论 Ig M型不规则抗体是引起ABO血型正反定型不相符的主要原因,高效价的Ig G型不规则抗体也可引起ABO血型正反定型不相符,在ABO血型鉴定中必须正反定型,同时进行不规则抗体筛选才能保证检测结果的准确性和输血安全。
[Abstract]:Objective to investigate the specificity of irregular antibodies and their immunoglobulin types which cause the incompatibility of positive and negative typing of ABO blood groups. Methods the ABO and Rh D blood groups of patients were identified by microcolumn gel method. The blood samples with different positive and negative typing of ABO blood group were re-examined by the traditional test tube method, and the irregular antibody was screened by microcolumn gel method, and the specificity was identified. Results among 56 cases of irregular antibodies that caused the inconsistency of positive and negative typing of ABO blood group, 19 cases were found to have MNS blood group system, 19 cases were found to have a 33. 9% P blood group system and 1 case was found to have a 1. 8% ABO blood group system. 15 cases were found to have a blood group system of 26. 8% and 4 cases of Rh blood group. The antibody titer of 10 cases was 17.9%, and that of cold autoantibody 7 cases was 12.55.The immunoglobulin type was Ig M type in 42 cases. The antibody titer was 1 16: 1: 81922 Ig G type in 10 cases, and the antibody titer was 1: 1281% 1024Ig mg G mixed type 4 cases. The titer of antibody was 1: 3232: 1 256.37 鈩，

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