无偿献血者抗-HCV筛查与RIBA补充实验情况的综合分析

发布时间：2018-03-03 19:39

  本文选题：献血者　切入点：抗-HCV　出处：《中国输血杂志》2016年06期 　论文类型：期刊论文

【摘要】：目的评估献血人群抗-HCV ELISA试剂的初筛效果、重组免疫印迹试验(RIBA补充实验)和HCV RNA核酸检测的情况,以探索无偿献血人群抗-HCV的筛查效果。方法收集2013年5月31日-2015年1月20日118 350例标本,采用2个不同厂家抗-HCV ELISA初筛试剂检测,初筛有反应性的标本(S/CO≥0.75)采用RIBA的方法进行补充实验,RIBA补充实验不确定结果的标本进行核酸检测。结果 2种初筛酶免试剂检测结果的阳性符合率为64.6%,阴性符合率为99.9%,总符合率为99.9%。北京万泰抗-HCV抗体诊断试剂盒检测阳性标本93.3%分布于S/CO≥10.0以上,上海科华抗-HCV抗体诊断试剂盒检测阳性标本91.3%分布于S/CO≥7.0以上。2种初筛试剂单边阳性的结果中,RIBA补充实验总阳性结果的比例为2.7%,不确定结果的比例为18.4%,阴性结果的比例为78.9%,北京万泰试剂单边阳性对应有2例RIBA阳性结果,上海科华试剂单边阳性对应有1例RIBA阳性结果,2种试剂检测性能具有一定的互补性。初筛单边阳性及RIBA补充实验结果为不确定的标本核酸检测均为阴性。RIBA阳性标本条带分析结果中,Core、NS3、NS4.1、NS4.2、NS5条带所占的比例分别为35.4%、33.9%、14.9%、2.4%、13.4%;RIBA不确定结果中,仅有Core、NS3 2种条带,比例为49.2%、50.8%。结论在献血人群中抗-HCV ELISA试剂检测存在生物学上的假阳性,一定程度上造成血液资源的浪费,选择灵敏度高、特异性好的初筛试剂,辅以RIBA补充实验,对于保证输血安全具有非常重要的意义。
[Abstract]:Objective to evaluate the screening effect of anti-HCV ELISA reagent, recombinant Western blotting test (RIBA-supplementation test) and detection of HCV RNA nucleic acid in blood donors. Methods 118,350 samples from May 31st 2013 to January 20th 2015 were collected and tested by two different anti-HCV ELISA screening reagents. RIBA method was used to detect nucleic acid in samples with uncertain results of RIBA supplementation experiment. Results the positive coincidence rate of the results of the two enzyme immunoassay was 64.6 and the negative rate was 64.6. The coincident rate was 99.9 and the total coincidence rate was 99.9.The positive specimens of Beijing Wantai anti-HCV antibody diagnostic kit were found to be above 10. 0 or more in S- / CO ratio. 91.3% positive samples of Shanghai Kehua Anti-HCV Antibody Diagnostic Kit were found in the results of S- / CO 鈮，

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