贵阳市2013—2015年手足口病患儿EV71病毒VP1、VP4基因特征分析

发布时间：2018-03-16 09:20

  本文选题：手足口病　切入点：肠道病毒型(EV)　出处：《中国公共卫生》2016年06期 　论文类型：期刊论文

【摘要】：目的分析贵州省贵阳市2013—2015年手足口病患儿肠道病毒71型(EV71)VP1和VP4基因特征,为手足口病疫苗的选用和临床诊治提供参考依据。方法采集2013年8月—2015年4月在贵阳市第五人民医院就诊的120例手足口病轻症患儿鼻咽拭子和(或)粪便标本进行EV71病毒VP1和VP4全序列的逆转录-聚合酶链反应(RT-PCR)扩增和测序,并与美国国立生物技术信息中心公布的EV71 A、B、C基因型代表株进行系统进化分析,根据进化分析结果对此2个片段随机抽取20例与其他地区相同基因型进行氨基酸位点变异分析。结果所有样本病毒株均为C4基因亚型,与2008年北京、上海、安徽以及2011年深圳分离株成簇,20例EV71病毒株在VP1和VP4氨基酸位点变异与以上城市流行毒株相比,其中12例样本EV7的VP1片段氨基酸序列293位丙氨酸(A)变异为丝氨酸(S),其余位点均只有1例样本发生A26T、N31D、N282S突变,而VP4片段只有1个样本发生K52R突变。结论贵阳市2013—2015年手足口病患儿的EV71流行株均属C基因型的C4亚型,VP1片段常见A293S、A26T、N31D和N282S突变,VP4片段偶有K52R突变。
[Abstract]:Objective to analyze the characteristics of enterovirus 71 (EV71) VP1 and VP4 gene in children with hand-foot-mouth disease (HFMD) from 2013 to 2015 in Guiyang, Guizhou Province. Methods from August 2013 to April 2015, 120 children with hand foot and mouth disease who were treated in Guiyang 5th people's Hospital were collected nasopharyngeal swabs and / or fecal specimens from August 2013 to April 2015 in order to provide reference for the selection, clinical diagnosis and treatment of hand-foot-mouth disease vaccine. EV71 virus VP1 and VP4 sequences were amplified and sequenced by reverse transcription-polymerase chain reaction (RT-PCR). The phylogenetic analysis was carried out with the representative strain of EV71 Agna C genotype published by the National Biotechnology Information Center of the United States. According to the results of evolutionary analysis, 20 samples of these two fragments were randomly selected for amino acid locus variation analysis with the same genotype as other regions. Results all of the sample strains were C4 gene subtypes, which were similar to those of Beijing and Shanghai in 2008. In Anhui and Shenzhen in 2011, 20 strains of EV71 virus were clustered and mutated at the amino acid sites of VP1 and VP4, compared with the epidemic strains in the above cities. Among them, the amino acid sequence of VP1 fragment of EV7 in 12 cases was mutated into serine tassel, and only one of the other samples had a mutation of A26TX N31DN282S. However, K52R mutation was found in only one sample of VP4 fragment in Guiyang from 2013 to 2015. Conclusion K52R mutation is occasionally found in the C genotype C subtype C subtype VP1 fragment of A293SU A26TN 31D and N282S mutation VP4 in children with hand-foot-mouth disease in Guiyang from 2013 to 2015.
【作者单位】： 贵州医科大学检验学院;贵阳市公共卫生救治中心;
【分类号】：R725.1;R440
，

本文编号：1619286