氧自由基、HMGB1在脓毒症大鼠心肌组织中的动态表达及丹红注射液的干预影响

发布时间：2018-03-18 10:16

本文选题：脓毒症　切入点：心肌损伤　出处：《中华中医药学刊》2016年01期 　论文类型：期刊论文

【摘要】：目的:观察丹红注射液对脓毒症大鼠心肌损伤的保护作用并探讨其可能机制。方法:雄性Wistar大鼠分为6组:正常组、假手术组、模型6 h组、模型24 h组、丹红6 h组和丹红24 h组,每组各8只。盲肠结扎并穿孔(CLP)法建立脓毒症大鼠动物模型,观察各组心肌组织超微结构改变,心肌组织丙二醛(MDA)、超氧化物岐化酶(SOD)和高迁移率族蛋白1(HMGB1)含量的变化。结果:CLP术后,模型组大鼠心肌组织MDA含量、HMGB1蛋白合成进行性升高、SOD活力则持续降低,与假手术组比较有差异(P0.05),丹红组较模型组同时间点MDA、HMGB1及SOD均有改善,改善有统计学差异(P0.05);超微结构示模型组大鼠心肌细胞线粒体肿胀,部分肌纤维稀疏,至24 h时肿胀加重,部分空泡变性;丹红组心肌细胞损伤轻于模型组同时间点。结论:丹红注射液对脓毒症大鼠心肌损伤有一定程度的保护作用,其机制可能与清除氧自由基,降低HMGB1含量有关。
[Abstract]:Objective: to observe the protective effect of Danhong injection on myocardial injury in septic rats and explore its possible mechanism. Methods: male Wistar rats were divided into 6 groups: normal group, sham operation group, model group for 6 h, model group for 24 h. The sepsis rat model was established by cecal ligation and perforation with 8 rats in each group for 6 h and 24 h group respectively. The ultrastructure of myocardium was observed in each group. The changes of myocardial malondialdehyde (MDA), superoxide dismutase (SOD) and high mobility group protein (1HMGB1) were observed. Compared with sham-operated group, Danhong group improved MDAHMGB1 and SOD at the same time point and improved P0.05.The ultrastructure showed that myocardial mitochondria were swollen and some muscle fibers were sparse in the model group, and the swelling was aggravated at 24 h, and the changes of MDAHMGB1 and MDAHMGB1 in the model group were more severe than those in the control group. Partial vacuolar degeneration, myocardial cell damage in Danhong group was lighter than that in model group at the same time point. Conclusion: Danhong injection has some protective effect on myocardial injury in septic rats, and its mechanism may be related to scavenging oxygen free radicals and decreasing HMGB1 content.
【作者单位】：浙江中医药大学附属第三医院;
【基金】：浙江省自然科学基金项目(LY12H15004) 浙江省中医药科技计划项目(2012ZB069) 浙江中医药大学科研项目(2010LX22);浙江中医药大学附属第三医院科研项目(ZS10CA15)
【分类号】：R459.7

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