基因多态性与行为学和睡眠的相关研究

发布时间：2018-03-20 17:20

本文选题：基因多态性　切入点：智力　出处：《西安电子科技大学》2015年硕士论文　论文类型：学位论文

【摘要】：近年来,基因多态性已成为研究领域中的热门。基因决定着生命的根本结构和功能,储存着生命的种族、血型、成长和凋亡过程的全部信息。因此研究基因多态性具有非常重要的意义。本文旨在讨论儿茶酚胺氧甲基转移酶(COMT)、脑源性神经营养因子(BDNF)、细胞黏附分子和信号受体(NPTN)和腺苷A2A受体(A2A)四种基因的多态性。通过认知行为学(包括智力和记忆力)和睡眠纺锤波来研究基因多态性对人体表现行为的影响。论文以基因多态性、智力测试、记忆力测试和睡眠的理论为研究背景,概述它们的基本概念。从智力、记忆力和睡眠三个方向分析基因多态性对人体的影响。通过血液采样获得被试的基因,请专业公司分析出其基因型。根据韦氏智力量表、记忆量表对被试进行智力测试和记忆力测试,获得智力和记忆力结果。对于睡眠EEG数据,研究睡眠结构、睡眠阶段以及纺锤波提取,重点研究纺锤波的密度、平均频率、平均幅度和平均持续时间于基因多态性的相关性。通过对100名满足条件的被试采集5ml血液获得基因样本。根据基因多态性进行分组,对他们进行同样的智力测试、记忆力测试和睡眠实验。利用22.0版本的SPSS对结果进行单因素ANOVA分析,再对显著性结果进行事后检验,得出具体的基因型对人体的影响。结果表明,基因多态性与认知行为的研究中,BDNF(rs6265)的A型纯合子与携带G基因的组合在记忆测试中的联想(P=0.04)和触摸(P=0.02)有着显著性差异,AG基因型和GG基因型在智力测验中的知识分测验有着显著性差异,P=0.03;NPTN(rs7171755)的A型纯合子与G型纯合子在智力测验中的数字广度分测验有着显著性差异,P=0.03。在基因多态性与睡眠纺锤波的研究中,发现COMT(rs4680)的AG杂合子与G型纯合子在每个电极上的纺锤波平均频率有着显著性差异,P0.05;BDNF(rs6265)在左额叶的纺锤波平均持续时间上,AA型与GG型表现出显著性差异(P=0.03),AG型与GG型表现出显著性差异(P=0.03);NPTN(rs7171755)的AA型与AG型在右额叶的纺锤波密度上表现出显著性差异,P=0.02,在中间的中央区域的纺锤波密度上也表现出显著性差异,P=0.04;ADORA2A(rs5751876)的CT杂合子和T纯合子与左侧中央区域的纺锤波持续时间有着显著性差异,P=0.03。本文的结果表明COMT(rs4680)、BDNF(rs6265)、NPTN(rs7171755)和ADORA2A(rs5751876)四种基因的多态性对人体的智力、记忆力和睡眠可能是有作用的。本研究结论提高了我们对基因多态性的进一步认识,某种程度上为基因多态性的研究提供了理论支持。
[Abstract]:In recent years, gene polymorphism has become a hot research field. Genes determine the fundamental structure and function of life, and store the race and blood type of life. Therefore, the study of gene polymorphism is of great significance. This paper aims to discuss catecholamine-methyltransferase (COMTT), brain-derived neurotrophic factor (BDNF), cell adhesion molecules and signal receptors. Polymorphisms of four genes (NPTNA) and adenosine A2A receptor (A2A). The effects of gene polymorphisms on human behavior were studied by cognitive behavior (including intelligence and memory) and sleep spindles. Intelligence test, memory test and sleep theory are the background of this study. Their basic concepts are summarized. The effects of genetic polymorphisms on human body are analyzed in three directions: intelligence, memory and sleep. According to Wechsler Intelligence scale, memory scale, intelligence test and memory test, intelligence and memory results were obtained. For sleep EEG data, sleep structure was studied. Sleep stage and spindle wave extraction, focusing on the density and average frequency of spindles, The average amplitude and mean duration were correlated with genetic polymorphism. The same intelligence tests were performed on 100 subjects who met the criteria by taking 5ml of blood samples and grouping them according to the genetic polymorphism. Memory test and sleep test. Single factor ANOVA analysis of the results was carried out using version 22. 0 SPSS, and the significant results were tested afterwards. The effects of specific genotypes on the human body were obtained. Genetic Polymorphism and Cognitive behavior in the study of genetic Polymorphism and Cognitive behavior, there were significant differences in the knowledge of genotype A homozygote and carrying G gene combination in memory test (P0. 04) and Touch Pu (0. 02)) in intelligence test of AG genotype and GG genotype. There was a significant difference in the number span subtest between type A homozygote and type G homozygote in intelligence test. In the study of gene polymorphism and sleep spindles, there was a significant difference in the number span subtest between the A type homozygote and G type homozygote. It was found that the average frequency of AG heterozygote and G-type homozygote on each electrode was significantly different (P0.05BDNFN rs6265). There was significant difference between AA type and GG type in the mean duration of spindles in left frontal lobe. There is significant difference between AA type and AG type in the density of spindles in the right frontal lobe, and in the central region of the middle area, there is also a significant difference in the density of spindles in the central region of P0.04ADORA2Atrs 5751876). The CT heterozygotes and T homozygotes are also different from each other in the right frontal lobe (P0.04ADORA2Agnrs5751876), and in the central region of the middle part, there is also a significant difference in the density of the spindles in the central region (P0.04ADORA2Atrs5751876). There is a significant difference in the spindles duration in the left central region (P0.03.The results show that the polymorphism of four genes, COMTR rs4680, BDNF rs6265) and ADORA2An rs5751876, have intelligence effects on human intelligence. Memory and sleep may play a role in this study. The results of this study improve our understanding of gene polymorphism and to some extent provide theoretical support for the study of gene polymorphism.
【学位授予单位】：西安电子科技大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R740

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