职业性猪接触与人群携带金黄色葡萄球菌的关系研究

发布时间：2018-03-22 11:59

本文选题：金黄色葡萄球菌　切入点：耐药性　出处：《广东药学院》2015年硕士论文　论文类型：学位论文

【摘要】：目的探讨珠三角地区职业性猪接触与人群携带金葡菌的流行水平,携带的影响因素,耐药情况以及分子分型特征,并进一步了解人群携带动物源性金葡菌的现状,为更好地为制定预防控制金葡菌的策略和措施提供科学依据。方法采用方便抽样的方法对深圳、东莞、江门和佛山4个地区的591名职业人群及843名对照人群进行问卷调查及鼻拭子样本收集,结合传统生化实验及PCR方法对收集的样本进行金葡菌的分离鉴定,采用Kirby-Bauer(K-B)纸片扩散法对分离的金葡菌进行抗生素敏感性检测。应用普通PCR方法检测pvl毒力基因;采用多重PCR方法对MRSA进行SCCmec分型;应用多位点序列分型对金葡菌进行分子分型分析。采用χ2检验及Fisher确切概率法和非条件的Logistic回归分析方法分析研究对象携带金葡菌及MRSA的影响因素。结果591名职业人群共分离出80株金葡菌(13.5%),MRSA 43株(7.5%);843名对照人群共分离出97株金葡菌(11.5%),MRSA 16株(1.9%);职业人群MRSA的携带率明显高于对照人群(P0.05)。耐药性分析发现MRSA对12种常用抗生素的耐药性明显高于MSSA(P0.05),并且发现职业人群携带的金葡菌对抗生素的耐药性和多重耐药率明显高于对照人群。本次分离的177株金葡菌共检测出3株携带PVL毒素,携带率(1.7%)较低,但均来自职业人群。SCCmec分型发现此次分离的MRSA主要为IV型和V型;而MLST分型共发现了30种ST型别,并在职业人群中检出了16株ST9型亚洲流行的动物源性金葡菌。影响因素分析发现30岁年龄组携带MRSA的风险是45年龄组的2.75倍,30-45岁年龄组携带MRSA的风险是45年龄组的2.56倍。结论1.职业人群MRSA的携带率明显高于对照人群。2.职业人群携带的金葡菌耐药程度和多重耐药率明显高于对照人群。3.本研究分离的金葡菌PVL毒素的携带率较低,但均来自职业人群。4.职业人群中携带了亚洲流行的动物源性金葡菌。5.本研究对象携带MRSA的影响因素主要与年龄相关。6.职业人群与对照人群携带的金葡菌亲缘关系较远。
[Abstract]:Objective to investigate the prevalence level of Staphylococcus aureus carrying in occupational pigs and population in Pearl River Delta region, the influencing factors of carrying Staphylococcus aureus, drug resistance and molecular typing characteristics, and to further understand the current situation of carrying Staphylococcus aureus from animals in the population. In order to provide a scientific basis for formulating strategies and measures for the prevention and control of Staphylococcus aureus. 591 professional population and 843 control group in Jiangmen and Foshan were investigated by questionnaire and collected nasal swabs. Staphylococcus aureus was isolated and identified by traditional biochemical experiment and PCR method. The susceptibility of isolated Staphylococcus aureus to antibiotics was detected by Kirby-Baueren K-B-based disk diffusion method, pvl virulence gene was detected by ordinary PCR method, and SCCmec typing of MRSA was performed by multiple PCR method. The molecular typing of Staphylococcus aureus was analyzed by multilocus sequence typing. The influencing factors of staphylococcus aureus and MRSA were analyzed by 蠂 2 test, Fisher exact probability method and non-conditional Logistic regression analysis. A total of 80 strains of Staphylococcus aureus were isolated from the industrial population. A total of 97 strains of Staphylococcus aureus MRSA43 strains were isolated from 843 control subjects, and the carrying rate of MRSA in the occupational population was significantly higher than that in the control group (P0.055.The drug resistance analysis showed that MRSA was resistant to 12 common antibiotics). The drug resistance and multidrug resistance of Staphylococcus aureus carried by professional population were significantly higher than those of control group. Three strains of Staphylococcus aureus were detected to carry PVL toxin. The carrying rate was low, but all of them were from the occupational population. SCCmec typing found that the MRSA isolates were mainly type IV and V type, and 30 St types were found in MLST typing. The risk of carrying MRSA in 30 years old group was 2.75 times higher than that in 45 years old group, and the risk of carrying MRSA in 30-45 years old group was 45 years old, and the risk of carrying MRSA in 30 to 45 years old group was 2.75 times higher than that in 45 years old group, and 16 strains of animal origin staphylococcus aureus were detected in occupational population. Conclusion 1.The carrying rate of MRSA in occupational population was significantly higher than that in control group. The drug resistance and multidrug resistance rate of Staphylococcus aureus in occupational population was significantly higher than that in control group .3.The carrying rate of PVL toxin of Staphylococcus aureus isolated in this study was lower than that in control group. However, all of them were from occupational population. 4. In the occupational population, the prevalence of MRSA in Asia was found. 5. The influencing factors of carrying MRSA were mainly related to age. 6. The relationship between occupational population and control group was far from that of Staphylococcus aureus.
【学位授予单位】：广东药学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R446.5

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