外周血循环滤泡辅助性T细胞及IL-21在银屑病中的表达及意义

发布时间：2018-04-01 15:59

本文选题：银屑病　切入点：滤泡辅助T细胞　出处：《中国免疫学杂志》2016年11期

【摘要】：目的:探讨外周血循环滤泡辅助性T细胞(Follicular helper T cells,Tfh)及相关细胞因子IL-21在银屑病患者中的表达水平及其与疾病活动度的关系。方法:收集38例银屑病患者及32例健康对照者,流式细胞术检测外周血循环CD4~+CXCR5~+Tfh和CD4~+CXCR5~+ICOS~+Tfh细胞比例以及Th17细胞比例;ELISA检测血清IL-21浓度;分析这些指标间及与银屑病疾病活动度评分PASI间的相关性。结果:与健康对照者相比,银屑病患者外周血循环CD4~+CXCR5~+Tfh和CD4~+CXCR5~+ICOS~+Tfh细胞比例更高,血清IL-21浓度和Th17细胞比例亦显著高于对照组(P0.05);IL-21浓度与CD4~+CXCR5~+Tfh和CD4~+CXCR5~+ICOS~+Tfh细胞比例均显著正相关,而与Th17细胞比例间无显著相关性(P0.05);且CD4~+CXCR5~+ICOS~+Tfh细胞比例和血清IL-21与银屑病疾病活动度PASI评分显著正相关,而CD4~+CXCR5~+Tfh则与之无显著相关性(P0.05)。结论:银屑病患者外周血循环Tfh比例、IL-21浓度上调与银屑病患者疾病活动度密切相关,提示Tfh可能参与银屑病的发生发展过程,这一效应可能是通过分泌高水平IL-21实现;IL-21浓度与Tfh细胞比例相关,而与Th17细胞比例无相关性提示银屑病患者IL-21可能主要由Tfh细胞分泌。
[Abstract]:Objective: to investigate the expression level of peripheral blood circulating follicular helper T cell (T cell) and related cytokine IL-21 in patients with psoriasis and its relationship with disease activity. Methods: 38 patients with psoriasis and 32 healthy controls were collected. The percentage of CD4 ~ CXCR5 ~ Tfh and CD4 ~ CXCR5 ~ ICOS- Tfh cells and the proportion of Th17 cells in peripheral blood circulation were detected by flow cytometry. The serum IL-21 concentration was detected by Elisa. Results: compared with healthy controls, peripheral blood circulation CD4 ~ CXCR5 ~ Tfh and CD4 ~ CXCR5 ~ ICOS- Tfh cells were higher in psoriatic patients. The concentration of serum IL-21 and the proportion of Th17 cells were significantly higher than those of the control group (P 0.05). The concentration of IL-21 was positively correlated with the proportion of CD4 ~ CXCR5 ~ Tfh and CD4 ~ CXCR5 ~ ICOS- Tfh cells. The ratio of CD4 ~ CXCR5 ~ ICOS- Tfh cells and serum IL-21 were positively correlated with the PASI score of psoriatic disease activity. There was no significant correlation between CD4 ~ CXCR5 ~ Tfh and P0.050.Conclusion: the increase of circulating Tfh ratio and IL-21 concentration in peripheral blood of psoriasis patients are closely related to the disease activity of psoriasis patients, suggesting that Tfh may be involved in the occurrence and development of psoriasis. This effect may be related to the proportion of Tfh cells by secreting a high level of IL-21, but not to the proportion of Th17 cells, suggesting that IL-21 may be mainly secreted by Tfh cells in psoriatic patients.
【作者单位】：九江市第一人民医院/南昌大学附属九江医院检验科;江西省医学科学院分子医学研究所;南昌大学免疫与生物治疗研究所;
【基金】：国家自然科学基金(No.81160056)
【分类号】：R758.63;R446.6

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