5种抗菌药物体外抗厌氧菌活性研究

发布时间：2018-04-06 20:21

本文选题：厌氧菌　切入点：最低抑菌浓度　出处：《中国感染与化疗杂志》2016年06期

【摘要】：目的研究氨苄西林-舒巴坦、阿莫西林-克拉维酸、厄他培南、甲硝唑和克林霉素对临床多中心标本分离厌氧菌的抗菌活性。方法对179株厌氧菌进行16S rRNA基因测序鉴定。药敏试验S每糃LSI M11-A8文件,采用琼脂稀释法测定5种抗菌药物对179株临床分离厌氧菌的最低抑菌浓度(MIC)。结果77株革兰阴性厌氧杆菌中,56株拟杆菌属细菌对厄他培南敏感率最高,为96.4%,对克林霉素敏感率为39.3%;21株革兰阴性厌氧杆菌对氨苄西林-舒巴坦全敏感。43株革兰阳性厌氧杆菌和54株革兰阳性厌氧球菌,对氨苄西林-舒巴坦和厄他培南的敏感率较高(87.5%),对克林霉素的耐药率较高。结论氨苄西林-舒巴坦、阿莫西林-克拉维酸、厄他培南对临床常见厌氧菌保持较高的抗菌活性,不同菌属细菌对甲硝唑耐药率差异较大,对克林霉素耐药率较高。
[Abstract]:Objective to study the antibacterial activity of ampicillin-sulbactam, amoxicillin-clavulanic acid, ertapenem, metronidazole and clindamycin against anaerobes isolated from multicentre clinical specimens.Methods the 16s rRNA gene was sequenced from 179 strains of anaerobic bacteria.The minimal inhibitory concentration of 5 antimicrobial agents against 179 clinical strains of anaerobes was determined by Agar dilution method.Results among 77 Gram-negative anaerobic bacteria, 56 strains of Bacteroides were most sensitive to ertapenem.The sensitivity rate to clindamycin was 39.3%. 21 strains of gram-negative anaerobic bacilli were completely sensitive to ampicillin and sulbactam. 43 strains of gram-positive anaerobic bacilli and 54 strains of gram-positive anaerobic cocci were detected.The sensitivity to ampicillin-sulbactam and ertapenem was higher than that to clindamycin.
【作者单位】：中国医学科学院北京协和医院检验科中国医学科学院北京协和医学院研究生院;
【基金】：国家卫生计生委公益性行业科研专项(201402001)
【分类号】：R446.5

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